[用于法医毒理学的干血斑点分析系统的开发:对五种常见药物和毒素的案例研究]
Dong-Bin Su1, Lin-Pei Dong2, Yun-Feng Zhang2
1College of Investigation, People's Public Security University of China, Beijing 100038, China.
Se pu = Chinese journal of chromatography
|March 19, 2024
概括
干血斑点 (DBS) 技术为法医毒理学中药物分析提供了一种方便的方法. 这项研究验证了DBS用于量化五种药物,在室温下显示出高精度和稳定性,支持其在公共安全中的使用.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 生物医学科学 生物医学科学
背景情况:
- 干血斑点 (DBS) 技术简化了血液样本的采集,运输和储存.
- DBS在法医科学中越来越多地用于药物分析,但在中国缺乏用于司法用途的标准化和验证.
- 在DBS准备,储存和分析中的变化可以显著影响结果.
研究的目的:
- 开发和验证一种超高性能液体染色学-并联质谱法 (UHPLC-MS/MS) 方法,用于在DBS中量化五种药物.
- 调查DBS样品的最佳准备,预处理,分析和储存条件.
- 评估DBS样品的稳定性和可靠性与全血相比,用于法医药物分析.
主要方法:
- 开发了一种UHPLC-MS/MS方法,用于定量检测甲基胺,利多卡因,胺,芬太尼和亚泽.
- 优化了DBS制备,溶剂提取和分析条件 (移动相,梯度).
- 评估了储存条件 (温度,湿度,密封) 在60天内对DBS稳定性的影响.
主要成果:
- 对于五种向药物,确立了强烈的线性 (r^2:0.99830.9997) 和灵敏度 (LOD:0.20.5 ng/mL).
- DBS样本与全血具有很高的定量一致性 (平均差异<8%).
- 目标物质在室温下在DBS中保持60天的稳定性,不需要特殊的储存条件.
结论:
- 经过验证的UHPLC-MS/MS方法适合在DBS中可靠量化目标药物.
- DBS技术为法医毒理学中的血液样本保存提供了一个稳定,方便和定量矩阵.
- 在公共安全应用中,DBS为药物和毒素分析提供了一个轻量级和实用的解决方案.
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