全基因组前置基因查,以确定调解纳米粒子吸收和细胞内处理的受体和蛋白质
Daphne Montizaan1, Roberta Bartucci1, Catharina Reker-Smit1
1Department of Nanomedicine & Drug Targeting, Groningen Research Institute of Pharmacy (GRIP), University of Groningen, Groningen, The Netherlands.
Nature nanotechnology
|March 20, 2024
概括
基因查发现了细胞纳米粒子吸收的新途径. 这项研究表明,低密度脂蛋白受体 (LDLR) 和食尸体受体SCARB1,以及硫酸,介导纳米粒子积累和贩运.
科学领域:
- 细胞生物学 细胞生物学
- 纳米技术纳米技术
- 遗传学 是一个遗传学.
背景情况:
- 纳米粒子的细胞处理对纳米医学至关重要.
- 描述这些途径是复杂的,特别是与非正典机制.
研究的目的:
- 通过基因查发现参与纳米颗粒细胞内积累的受体和蛋白质.
- 了解人类血清冠状体在纳米粒子吸收中的作用.
主要方法:
- 使用插入性突变发生的全基因组前置遗传查.
- 用减少纳米粒子积累的分类细胞.
- 深度测序以识别丰富的基因.
主要成果:
- 确定了80个涉及纳米粒子细胞内积累的丰富基因.
- 证实低密度脂蛋白受体 (LDLR) 和食尸体受体SCARB1介导吸收.
- 发现的肝硫酸盐作为一种特定的纳米粒子受体.
- 发现一些目标会影响纳米颗粒到 lysosomes 的运输.
结论:
- 基因查是描述纳米粒子路径的强大工具.
- 冠状涂层纳米粒子通过多个受体内化.
- 确定了影响纳米粒子细胞处理的新型受体和途径.
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