雷多克斯后翻译性修饰的病理影响
Charbel Chahla1, Hervé Kovacic1, Lotfi Ferhat1
1Faculté de Médecine, INP, Institut de neurophysiopathologie, Aix Marseille Université, CNRS, Marseille, France.
氧化应激会导致影响疾病的蛋白质修饰. 虽然全球抗氧化疗法可能会失败,但针对特定的氨酸或氨酸残留物通过调节蛋白质活性提供了潜在的治疗益处.
科学领域:
- 生物化学和分子生物学
- 细胞再氧化生物学 细胞再氧化生物学
背景情况:
- 由活性氧物种 (ROS) 驱动的氧化应激会诱导蛋白质,核酸和脂质的临界氧化还原后翻译性修饰 (PTMs).
- 这些修改,包括囊和氨酸氧化和蛋白质碳化,与各种病理有关.
- 了解这些氧化还原诱导的PTM对于破译疾病机制至关重要.
研究的目的:
- 在病理学背景下审查最近关于氨酸和氨酸氧化和蛋白质碳化的数据.
- 探索氧化还原诱导PTMs对蛋白质功能,活性和疾病发展的影响.
- 评估针对特定的氧化还原修饰的治疗潜力.
主要方法:
- 关于氧化应激和氧化还原PTMs近期进展的文献评论.
- 分析详细介绍ROS和反应性物种 (RNS) 对蛋白质功能的影响的研究.
- 检查氧化的选择性和其对蛋白质结构和环境的依赖性.
主要成果:
- 氧化应激导致各种PTM,数百种蛋白质被确定为目标.
- 红氧诱导的PTM对蛋白质功能表现出复杂的影响,其中一些修改降低了活性,而另一些则增加了活性.
- 囊二醇和氨酸残留物的氧化可以是可逆的或不可逆的,而碳基化是不可逆的.
结论:
- 复杂的,取决于环境的蛋白质功能由氧化还原PTMs调节表明,广泛的抗氧化疗法可能是无效的.
- 针对治疗干预的特定的氨酸或氨酸残留物有望调节蛋白质活性和治疗疾病.
- 开发用于精确向这些残留物的药理工具是未来治疗发展的关键领域.
更多相关视频
10:24Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
Published on: June 7, 2018
06:10Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
Published on: June 18, 2020
相关概念视频
Protein Modifications in the RER
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
Covalently Linked Protein Regulators
These groups modify specific amino acids in a protein....
Electron Transport Chain: Complex I and II
ROS generation is regulated and maintained at moderate levels necessary...
Phosphorylation
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
Oxidation and Reduction of Organic Molecules
The removal of an electron from a molecule, results in a...
Regulated Protein Degradation
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
