在猪化过程中选择的副本数变异从全基因组再测序中推断出来
Wei Zhang1, Chengliang Xu1, Mei Zhou1
1Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Anhui Provincial Breeding Pig Genetic Evaluation Center, Key Laboratory of Pig Molecular Quantitative Genetics of Anhui Academy of Agricultural Sciences, Anhui Provincial Key Laboratory of Livestock and Poultry Product Safety Engineering, Hefei, China.
Frontiers in veterinary science
|March 20, 2024
概括
副本数变异 (CNV) 分析揭示了万北猪的关键遗传特征. 这项研究确定了164种与繁殖,生长和免疫相关的精选CNV,有助于品种的保存和未来发展.
科学领域:
- 动物遗传学动物遗传学
- 基因组学就是基因组学.
- 畜牧科学 畜牧科学 畜牧科学
背景情况:
- 中国拥有多种多样的猪品种,这些品种是通过广泛的选择开发出来的.
- 了解生殖质的特征对于养猪品种的保护和改进至关重要.
- 拷贝数变异 (CNV) 是猪资源研究中与单核酸多态 (SNP) 相比较的研究不足的遗传标记.
研究的目的:
- 为了研究万北猪 (WBP) 的复制数变异 (CNV).
- 为了识别选择中的CNV及其相关基因.
- 为万北猪养殖和保存提供基因组基础.
主要方法:
- 在18只万北猪的全基因组重新测序中.
- 对副本编号变异 (CNVs) 的识别和描述.
- 固定指数 (Fst) 分析以检测选定的CNV.
- 在选定的CNV中对基因的功能丰富分析.
主要成果:
- 在WBP中确定了8,783个CNV (30.07Mb),包括8,427个删除和356个重复.
- 在前1%的Fst值中检测到164个CNV,表明选择.
- 关联选择的CNV与参与生殖 (例如SPATA6),生长 (例如NR6A1) 和免疫 (例如PARD3) 的基因.
结论:
- 这项研究通过CNV分析阐明了万北猪的基因组特征.
- 识别和选择的CNV提供了对适应性特征的见解.
- 这些发现为北猪的有针对性的繁殖策略提供了理论基础.
相关概念视频
Comparing Copy Number Variations and SNPs
17.7K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.7K
Single Nucleotide Polymorphisms-SNPs
15.1K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.1K
Incomplete Dominance
22.5K
Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.
22.5K
Multi-species Conserved Sequences
3.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.9K
Next-generation Sequencing
88.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.8K
Evolutionary Relationships through Genome Comparisons
5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K


