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主要的肉症与高水平的自发NET形成有关
Irina Balazs1,2, Manuel Stelzer1, Julia Traub3
1Department of Internal Medicine, Division of Gastroenterology and Hepatology, Medical University of Graz, Graz, Austria.
Frontiers in cell and developmental biology
|March 20, 2024
概括
自发性中性粒细胞外细胞陷 (NET) 形成在肉症患者中升高,可能导致炎症. 然而,对细菌刺激和中性粒细胞细胞形成的NET形成反应在肉症中保持不变,即使是肝硬化.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 胃肠病学 胃肠病学
背景情况:
- 肉,肌肉损失,是肝硬化的一个常见并发症,也可以独立发生.
- 中性粒细胞的功能,包括中性粒细胞外细胞陷 (NET) 形成,与慢性炎症有关,但在肉症中未得到充分研究.
研究的目的:
- 在患有肉类症,肝硬化或不患肝硬化的患者中研究中性粒细胞功能变化 (细胞,反应性氧物种 (ROS) 生产,NET形成).
主要方法:
- 从92名患者中分离出了中性粒细胞 (带有缩症的肝硬化,没有缩症的肝硬化,仅有缩症) 和10名健康对照.
- 中性粒细胞的功能通过刺激大肠杆菌,博12-米里酸 (PMA) 或化介质来评估.
- 使用DNA区域和NETosis分析 (DANA) 算法量化了NET形成;使用商业套件测量了细胞和ROS产量.
主要成果:
- 自发的NET形成在单独患有皮病的患者中明显高于患有肝硬化和皮病的患者,以及健康对照患者.
- 与对照组相比,在所有患者组 (肝硬化,带有肉的肝硬化,肉症) 中,响应PMA的NET形成减少.
- 中性细胞化和ROS产量在患者组和健康对照组之间没有显著差异.
结论:
- 萨尔科佩尼亚的高自发NET形成可能会导致慢性炎症,但不会损害抗菌防御机制.
- 中性细胞化和ROS产量在肉症患者中没有显著影响.
- 在临床研究中,DANA算法是分析NET形成的一个可行的工具,它表明了sarcopenia中基于NET形成的生物标志物的潜力.
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