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在Drosophila中,嗅觉受体基因的外离子剪接

Xueying Shang¹, Gaëlle J S Talross¹, John R Carlson¹

¹Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511.

Proceedings of the National Academy of Sciences of the United States of America

|March 20, 2024

概括

研究人员在Drosophila气味受体 (或) 基因中发现了外子,这些基因可以被分割出来改变蛋白质. 这种在Or88a,一种激素受体中的脱离子拼接被保存,并可能通过减少功能性Or转录来调节神经元活动.

科学领域:

分子生物学分子生物学
神经科学是一个神经科学.
遗传学是一个遗传学.

背景情况:

嗅觉受体 (或) 基因的适当表达对嗅觉系统的功能至关重要.
替代拼接和多化是已知的调节基因表达的机制.

研究的目的:

研究Drosophila气味受体基因中的替代拼接事件,特别是外.
探索在Or88a中脱离子拼接及其跨物种的保护的功能影响.
了解神经元活动如何影响或基因表达和异型水平.

主要方法:

在39个Drosophila Or基因中识别和分析外子.
专注于Or88a,研究出离子拼接的保护和调节.
利用光遗传学和费洛蒙刺激来评估神经元活动对拼接的影响.

主要成果:

在39个研究的Drosophila Or基因中,在4个基因中发现了外子.
在Or88a中,一个激素受体,在五种Drosophila物种中保留了exitron拼接.
神经元激活,特别是光遗传刺激,显著增加了exitron-spliced或转录.

结论:

在Or88a中的外离子拼接可以通过减少功能性Or转录来作为神经元调节的机制.
替代拼接和多化事件有助于调节Or的异型.
神经元活动可以动态调节嗅觉受体基因表达和异型水平.

关键词:

这种植物是Drosophila.走出门口的出入口嗅觉嗅觉是一种嗅觉.转录后监管的规定.拼接拼接拼接拼接

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