相关实验视频
Updated: May 3, 2026

A Sensitive Method to Quantify Senescent Cancer Cells
Published on: August 2, 2013
新型PET成像探测器用于在体内定量检测衰老
Xin Xiang1, Chuning Dong1, Lianbo Zhou1
1Department of Nuclear Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, China.
我们开发了一种新的PET成像探头[68Ga]Ga-BGal,用于实时检测细胞衰老. 这个探测器在体内准确地可视化与衰老相关的β-galactosidase,为临床翻译提供了潜力.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 分子成像学分子成像学
- 放射化学 放射化学是指辐射化学.
背景情况:
- 检测细胞衰老对于疾病诊断和治疗监测至关重要.
- 目前用于检测细胞衰老的方法缺乏实时体内能力.
- 与衰老相关的β-galactosidase (SA-β-Gal) 是细胞衰老的一个关键生物标志物.
研究的目的:
- 开发一种新型的正电子发射断层扫描 (PET) 成像探针,针对SA-β-Gal.
- 为了评估探测器的性能,实时在体内定量检测细胞衰老.
- 评估开发的PET探针的潜在临床适用性.
主要方法:
- 一种新的PET成像剂[68Ga]Ga-BGal.Gal的合成和表征.
- 在体外和体内验证探针对β-银酸酶的敏感性和特异性的验证.
- 在临床前模型中进行的PET成像研究,SA-β-Gal表达的水平各不相同.
主要成果:
- 对于[68Ga]Ga-BGal.高标签产量 (>90%) 和放射性化学纯度 (>95%) 是可以实现的.
- 该探头在体外和体内都显示出对β-Gal检测的高灵敏度和特异性.
- PET成像成功地可视化了高SA-β-Gal表达的瘤,与探头吸收相关.
结论:
- [68Ga]Ga-BGal是一种稳定有效的PET成像探针,用于SA-β-Gal.
- 该探测器可以在临床前模型中实时体内定量检测细胞衰老.
- 这一发展对推进临床衰老成像有前途.
更多相关视频
09:45In Vivo Quantification of Protein Turnover in Aging C. Elegans using Photoconvertible Dendra2
Published on: June 13, 2020
08:56Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
Published on: July 12, 2022
相关概念视频
Positron Emission Tomography
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
Radiological Investigation III: Pulmonary Angiogram and PET Scan
Pulmonary Angiogram
A Pulmonary Angiogram is an invasive procedure involving injecting a contrast medium through a catheter threaded into the pulmonary artery or the right side of the heart to visualize the pulmonary vasculature. Computed Tomography (CT) scans have mainly replaced this...
Imaging Studies II: Positron Emission Tomography and Scintigraphy
Fundamental Principles of PET