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单细胞奥米克学将炎症信号识别为老鼠胚胎中的转分化触发器

Yifan Zhang¹, Zhixin Kang², Mengyao Liu³

¹Shandong Provincial Key Laboratory of Animal Cell and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, China.

Developmental cell

|March 20, 2024

概括

这项研究揭示了在发育过程中推动细胞命运变化的常见炎症信号. 鉴定出interleukin-33和Spi1是转基因分化过程中的关键调节剂,例如内皮转基因转基因转基因.

科学领域:

发展生物学发展生物学
细胞重编程细胞重编程
免疫学免疫学免疫学

背景情况:

转基因差异化,即直接细胞系转换,尚未完全理解,这限制了其治疗潜力.
确定控制细胞命运可塑性的普遍原则,对于推进再生医学和发育生物学至关重要.

研究的目的:

探索跨差异化的普遍原则.
描述不同细胞命运转化过程中的共同特征和分子触发因素.
研究炎症信号在发育压力诱导的细胞转换中的作用.

主要方法:

在小鼠胚胎中,单细胞转录组分析了内皮细胞转化为造血细胞的过渡 (EHT),内皮细胞转化为介质细胞的过渡以及上皮细胞转化为介质细胞的过渡.
对,转录因子表达和过渡信号的评分指数的应用.
综合转录基因和染色质可访问性数据的多模式分析.
宿命绘制分析.命运绘制分析.

主要成果:

确定过渡状态的共同特征,包括炎症特征.
发现了interleukin-33作为多重命运转换的触发器的保存作用.
证明炎症信号通过染色质可访问性和转录程序调节血液构造特征.
表明内皮特异性Spi1充当炎症效应因子,保护EHT.

结论:

常见的过渡状态和信号,特别是炎症途径,支持发育压力诱导的细胞命运转化.
介质素-33和Spi1是像EHT这样的转差分化过程中的关键调节剂.
单细胞奥米克为细胞可塑性的机制提供了强大的洞察力.

关键词:

从内皮转变为血液形成的转变.内皮细胞到介质细胞的过渡.从上皮细胞转变为介质细胞的转变.炎症信号传递是一种炎症信号传递.单细胞的奥米克.间是指一个间.通过差异化转化.

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