相关实验视频

Updated: Jun 30, 2025

Handling and Treatment of Male European Eels Anguilla anguilla for Hormonal Maturation and Sperm Cryopreservation

Handling and Treatment of Male European Eels Anguilla anguilla for Hormonal Maturation and Sperm Cryopreservation

Published on: January 28, 2018

在添加含有胆固醇的环极氨酸后,冷保存的Prochilodus lineatus精液的精子特性

K V Antunes¹, J J S Santos², I C S Carvalho²

¹Postgraduate Program in Animal Science, Center for Research in Pigs, Native and Wild Species, Federal University of Vale Do São Francisco, Petrolina, PE, Brazil; Department of Animal Science, Center for Research in Swine, Native and Wild Species, Federal University of Vale Do São Francisco, Petrolina, PE, Brazil.

|March 20, 2024

概括

在冷保存之前,在Prochilodus lineatus鱼精子中添加含胆固醇的循环德克斯特林 (CLC),可显著改善解后的精子运动性和膜完整性. 最佳的CLC度可提高水产养殖应用中的精子质量.

科学领域:

水产养殖和生殖生物学
米体的冷保存方法
鱼的精子质量评估鱼的精子质量评估

背景情况:

冷保存对于保存水产养殖中的遗传物质至关重要.
Prochilodus lineatus (Curimata) 的精子质量可能受到冷保存过程的影响.
荷载胆固醇的环氧素 (CLC) 正被研究为一种潜在的冷保护剂.

研究的目的:

为了评估不同胆固醇载荷环素 (CLC) 度对Prochilodus lineatus精子解后质量的影响.
确定最佳的CLC剂量,以改善这种物种中冷保存的精子参数.

主要方法:

从12个成年Prochilodus lineatus的精液在诱导精液后被收集.
精液被稀释并用CLC处理,其度从0到4.0毫克/120×10^6精子/毫升不等.
精子参数包括运动性,激活持续时间,寿命,血膜完整性和形态被评估在标准化冷保存和解协议后解后.

主要成果:

与对照组相比,用CLC治疗的精子表现出明显更高的运动性.
精子激活时间在用0.5,1.0和2.0毫克/毫升CLC治疗的样本中被延长.
在接受0.5,1.0,2.0和3.0毫克/毫升CLC治疗的精子中,血膜完整性得到增强,而4.0毫克/毫升组与对照组相比没有改善.

结论:

在冷保存前将0.5,1.0或2.0毫克/毫升的CLC添加到Prochilodus lineatus精液中,可以有效地改善精子运动性和血膜完整性.
这些发现表明,CLC是增强Prochilodus lineatus精子冷生存的有益添加剂,对水产养殖繁殖计划有影响.

关键词:

鱼类鱼类鱼类鱼类膜的完整性膜的完整性形态学形态学形态学移动性移动性移动性精子的长寿精子的长寿

更多相关视频

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit

Published on: December 12, 2011

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Published on: July 6, 2009

相关概念视频

Sperm Structure and Semen Composition

Sperm Structure and Semen Composition

During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...