来自Lactobacillus johnsonii的细胞外囊泡通过增强M2巨细胞两极分化来促进肠道屏障稳定
Shiyu Tao1, Jinping Fan1, Jingjing Li1
1College of Animal Sciences and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Journal of advanced research
|March 20, 2024
概括
益生菌衍生的细胞外囊泡 (EVs) 通过调节巨细胞功能来对抗腹性疾病. 这些发现为针对肠道炎症和疾病的新型益生菌疗法铺平了道路.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 腹性疾病对人类和动物的全球健康负担很大.
- 目前用于腹疾病的益生菌策略需要进一步开发,以便精确操纵.
研究的目的:
- 调查益生菌对巨细胞对抗腹疾病的分子机制.
- 阐明益生菌细胞外囊泡 (EVs) 如何影响巨细胞表型和肠道屏障功能.
主要方法:
- 在健康和腹的小猪身上对肠道微生物组进行了元基因组学分析.
- 便微生物移植 (FMT) 以确定肠道微生物和腹之间的因果关系.
- 使用益生菌干预 (Lactobacillus johnsonii及其EVs) 在ETEC K88感染小鼠中的体内研究.
- 在体外共培和转录组分析以确定分子机制.
主要成果:
- 大肠杆菌的丰富和Lactobacillus johnsonii的枯竭与小猪的腹相关.
- 便微生物群,而不是代谢物,传播了腹症状;L. johnsonii阻止了这种传播.
- L. johnsonii EVs调节了巨细胞表型,通过激活M2巨细胞和抑制NLRP3炎症酶激活来减少ETEC K88诱导的肠道炎症.
结论:
- 肠道微生物群的组成是腹疾病的关键驱动因素.
- 益生菌衍生的EVs通过调节巨细胞表型,有效地改善了腹疾病.
- 这些发现支持开发新的基于益生菌EV的治疗方法,用于治疗腹.
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