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脂酶和pH响应的双块共聚合物,含有碳和碳素贝他因,用于治疗耐美西林黄金葡萄球菌感染
Jipeng Xiao1, Meihui Yin1, Min Yang2
1College of Chemistry and Environment, Southwest Minzu University, Chengdu 610041, China; Precision Medicine Translational Research Center, West China Hospital, Sichuan University, Chengdu, 610041, China.
概括
新型化聚合物微粒与耐药细菌作斗争. 这些含有西普洛素的菌体显示出对抗抗甲素耐药性黄金葡萄球菌 (MRSA) 生物膜和浮游生物形式的增强有效性,提供了一个有前途的新疗法.
科学领域:
- 材料科学 材料科学 材料科学
- 聚合物化学 聚合物化学
- 纳米技术 纳米技术
背景情况:
- 耐多药细菌及其生物膜对公众健康构成重大威胁.
- 迫切需要创新的抗微生物策略来克服细菌耐药性.
研究的目的:
- 设计和合成具有可切换表面电荷的新型化聚合物微粒.
- 评估这些菌在浮游生物和生物膜状态下对抗抗甲素耐药黄金葡萄球菌 (MRSA) 的疗效.
- 研究化和表面电荷切换在增强抗微生物活性中的作用.
主要方法:
- 用RAFT聚合合成了聚合甲基酸二块共聚合物与碳链和碳素贝基.
- 两性共聚合物自组装成封装西普罗夫洛克萨的微粒 (CIP@FCBMs).
- 作为对照剂,制备了无小粒 (CIP@BCBMs);两种系统都在pH响应电荷,脂酶触发释放,稳定性,药物负载,细胞吸收以及在体外和体内抗菌/抗生物膜疗效方面进行了测试.
主要成果:
- 化 (CIP@FCBMs) 和无化 (CIP@BCBMs) 细胞表现出对pH反应的表面电荷和脂酶触发的药物释放.
- 由于化,CIP@FCBMs表现出优越的血清稳定性,更高的药物负载能力和增强的细胞吸收.
- CIP@FCBMs在体外和体内都显示出强大的抗菌和抗菌膜对MRSA的活性.
结论:
- 表面电荷可切换的化聚合物微粒对MRSA及其生物膜有效.
- 化通过提高稳定性和细胞吸收来提高纳米粒子性能.
- 这些工程纳米粒子为开发对细菌感染和生物膜的先进治疗提供了一个有希望的平台.
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