相关实验视频
Updated: Jun 30, 2025

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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
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循环RNA作为癌症疫苗新抗原的来源
Yi Ren1,2, Thamizhanban Manoharan1,2, Beijia Liu1
1Department of Pharmacy, National University of Singapore, Singapore.
Journal for immunotherapy of cancer
|March 20, 2024
概括
循环RNAs (circRNAs) 为结直肠癌 (CRC) 免疫疗法提供了一种新的新抗原来源,特别是在具有低突变负担的瘤中. 这项研究表明,来自circRNA的新表位细胞可以引起抗瘤T细胞反应并向癌细胞.
科学领域:
- 在瘤学瘤学.
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
背景情况:
- 基于体质新抗原的免疫疗法在具有低至中等突变负担的瘤中面临局限性.
- 微卫星稳定的结直肠癌 (CRC) 对当前的免疫疗法构成了挑战.
- 瘤关联的循环RNAs (circRNAs) 被探索为潜在的新表位的替代来源.
研究的目的:
- 调查瘤相关的circRNAs作为结直肠癌中新epitopes的来源的潜力.
- 识别和验证能够引起抗瘤免疫反应的circRNA衍生新抗原.
主要方法:
- 使用MiOncoCirc数据库和RNA测序识别与瘤相关的circRNAs.
- 通过定量实时PCR (RT-qPCR) 验证circRNA表达.
- 预测circRNA开放阅读框架的翻译潜力和HLA结合亲和力.
- 使用T细胞测定和患者衍生器官的免疫性和细胞毒性的功能验证.
主要成果:
- 在CRC样本中发现了circRAPGEF5和circMYH9的新表位,circMYH9在CRC样本中上调.
- 证明circRNA衍生的会引起抗原特异性T细胞反应和扩张.
- 用circMYH9培训的展示T细胞有效地消除了瘤有机体,但不是正常的有机体.
- 在液体活检中检测到circMYH9丰富,这表明了检测到疫苗接种的战略.
结论:
- 与瘤相关的circRNAs代表了用于癌症疫苗的新抗原的可行替代来源.
- 这种方法特别适用于向具有中度突变水平的瘤.
- 循环RNA为开发用于结直肠癌的新型免疫疗法提供了一个有希望的途径.
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