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Updated: Jun 30, 2025

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
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Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

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外部质量控制调查,用于识别微生物,使用矩阵辅助激光脱/离子化飞行时间质谱法

Kazuyuki Sogawa1, Azumi Fujinaga2, Hajime Okumura3

  • 1Department of Biochemistry, School of Life and Environmental Science, Azabu University.

Journal of microorganism control
|March 20, 2024
PubMed
概括

评估了使用矩阵辅助激光吸附/电离飞行时间质谱法 (MALDI-TOF MS) 进行细菌鉴定的外部准确性控制. 使用分数值开发的程序证明了在临床实验室的质量控制中有用.

关键词:
外部质量控制是外部质量控制.微生物的识别方法通过矩阵辅助的激光脱吸/电离.飞行时间质谱测量

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MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...
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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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