RNA结合蛋白可能调节增殖性糖尿病视网膜病变中细胞循环相关基因的替代拼接
Ning Yang1, Ningzhi Zhang1, Guojing Lu1
1Department of Ophthalmology, Renmin Hospital of Wuhan University, Wuhan, China.
Scientific reports
|March 21, 2024
概括
RNA结合蛋白 (RBPs) 通过改变基因表达来驱动多发性糖尿病视网膜病变 (PDR). 这项研究确定了关键的RBP及其与细胞循环途径有关的点,为PDR提供了新的治疗策略.
科学领域:
- 眼科医生 眼科 眼科
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- RNA结合蛋白 (RBPs) 通过替代拼接事件 (ASE) 参与了多发性糖尿病视网膜病变 (PDR) 的病原发生.
- 涉及PDR的特定RBP及其监管机制在很大程度上仍未被描述.
研究的目的:
- 在PDR新血管膜 (NVM) 和视网膜中识别差异表达的RBP和替代拼接基因 (ASG).
- 阐明RBP-AS监管网络在PDR病原体中的作用.
主要方法:
- 对公共转录组数据集GSE102485 (PDR与正常视网膜) 的分析.
- 在顶级RBPs和ASGs之间进行协同表达的选.
- 使用氧气诱导视网膜病变小鼠模型进行验证.
主要成果:
- 在PDR NVM和视网膜样本中确定了403个异常表达的RBP.
- 功能分析显示,ASG在细胞循环途径中的丰富.
- 构建了一个包含15个RBP及其目标ASG的RBP-AS监管网络,在小鼠模型中显著差异地表达SFPQ,MAP1B,HSP90AA1,MACF1和CCNH.
结论:
- 建立了一个新的RBP-AS交互网络,对PDR开发至关重要.
- 突出了细胞循环相关的ASEs在PDR中受到特定RBPs调节的作用.
- 提供了开发诊断和治疗策略的见解,以预防PDR相关的失明.
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