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一个宿主-微生物群互动体揭示了广泛的跨王国连接

Nicole D Sonnert^1,2, Connor E Rosen¹, Andrew R Ghazi^3,4

¹Department of Immunobiology, Yale School of Medicine, New Haven, CT, USA.

|March 21, 2024

概括

研究人员绘制了人类蛋白质与细菌之间的数百万个相互作用图, 这一发现揭示了微生物如何在分子层面影响人类健康和疾病.

科学领域:

微生物学
人类生理学
蛋白质组学

背景情况:

微生物对人类健康有深远的影响,
病原体和初生动物与人类蛋白质 (外蛋白质) 相互作用,以殖民和调节宿主生物学.
直接的外蛋白质组与微生物群的相互作用在很大程度上尚未被探索.

研究的目的:

开发和验证一种用于大规模评估人类外蛋白质组与微生物组相互作用的新技术.
探索宿主微生物相互作用的分子景观.

主要方法:

开发并验证了BASEHIT (细菌相关的分泌物与宿主相互作用技术).
调查了519种细菌菌株和3324种人类外蛋白之间的170多万个潜在相互作用.
分析结合模式以推断生物逻辑和功能角色.

主要成果:

发现了成千上万种新型宿主微生物相互作用的广泛互动组,其中包括383种细菌菌株和651种人类蛋白质.
观察到与细菌分化,组织起源和外蛋白特异性相关的特定结合模式.
鉴定了可能涉及利基殖民,组织重塑和免疫调节的菌株特异性相互作用.

结论:

揭示了人体外蛋白质组与居民微生物群之间的巨大,以前未被描述的分子相互作用网络.
证明不同的微生物相互作用形状与不同的宿主细胞和免疫系统影响相关.
表明这些分子相互作用对土著微生物在人类健康和疾病中的作用至关重要.

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