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综合的转录组和代谢组分析揭示了昆 (Chenopodium quinoa Willd.) 叶子颜色中的安托素生物合成

Min Zhang^1,2,3, Yueyou Li⁴, Junling Wang^1,2,3

¹North China Key Laboratory for Crop Germplasm Resources of Education Ministry, The Key Laboratory of Germplasm Resources of Hebei Province, Hebei Agricultural University, Baoding, Hebei Province, 071000, P. R. China.

BMC plant biology

|March 21, 2024

概括

昆叶的颜色变化是由安东氨酸合成驱动的,由基因表达变化解释. 这项研究为为农业和美学开发彩色华提供了洞察力.

科学领域:

植物科学植物科学
分子生物学分子生物学
农业科学农业科学

背景情况:

昆叶表现出多种多样的颜色,增强了景观美学.
在半干旱地区发展以休为导向的观光农业的潜力.

研究的目的:

研究绿色和粉红色菜叶中安托氨酸合成的分子机制.
使用集成的转录组和代谢组分析.

主要方法:

综合转录组和代谢组分析.
对差异表达基因 (DEG) 和差异积累代谢物 (DAM) 的分析.
定量实时PCR (qRT-PCR) 用于DEG验证.

主要成果:

黄和安东的生物合成途径与安东积累有显著的关联.
特定的基因表达模式 (例如,PAL,CHS,4CL,HCT,F3H,FLS) 和调控因素 (bHLH14,WRKY46,TGA) 与粉红色和绿色的叶子颜色相关.
确定了关键的酶和转录因子,调节了安东素水平.

结论:

在花叶中解明了花素生物合成的分子调节机制.
提供了发展休和观光农业的理论基础,采用多彩的菜品种.

关键词:

DAMs 的使用方式.DEGs 是一个DEG.综合转录组和代谢组分析.在TFs中,我们可以使用TFs.qRT-PCR 是一个很好的方法.

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