相关实验视频

Updated: Jun 30, 2025

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Published on: September 23, 2021

使用细胞外囊泡对抗抗原的向性细胞传递用于抗癌免疫疗法

Xuan T T Dang^1,2, Cao Dai Phung^1,2, Claudine Ming Hui Lim^1,2

¹Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore.

Cell proliferation

|March 21, 2024

概括

红细胞衍生的细胞外囊泡 (RBCEVs) 将癌症抗原传递给树突细胞 (DCs),增强T细胞激活,以改善癌症疫苗. 这个平台克服了当前细胞外囊泡 (EV) 疗法的局限性.

科学领域:

免疫学免疫学免疫学
生物技术是生物技术.
癌症研究癌症研究

背景情况:

细胞外囊泡 (EVs) 正在探索用于癌症疫苗抗原的输送.
目前的EV疗法面临着DNA污染和高生产成本等挑战.
红细胞衍生电动汽车 (RBCEV) 提供了一个潜在的替代平台.

研究的目的:

开发一种使用RBCEVs的癌症疫苗的新型抗原输送平台.
针对脏DEC-205阳性树突细胞 (DCs),以增强抗瘤免疫力.
评估RBCEVs载有卵泡素 (OVA) 的有效性,用于T细胞原始化.

主要方法:

卵蛋白 (OVA) 蛋白被装载到RBCEVs上.
RBCEVs被设计成一种反DEC-205抗体,用于有针对性的直流输送.
产后对MHC I类和T细胞激活的抗原呈现进行了评估.

主要成果:

RBCEVs成功地将OVA蛋白传递给DCs.
通过抗DEC-205工程的RBCEV进行向的输送,显著增强了DC抗原呈现.
这导致抗原反应性T细胞的强烈激活.

结论:

RBCEVs 作为癌症疫苗中抗原输送的有效平台.
用RBCEV准DEC-205+DCs可以提高T细胞的反应.
这种方法对在临床环境中开发个性化癌症疫苗充满希望.

更多相关视频

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

Published on: January 6, 2014

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

Published on: February 5, 2021

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Cancer Vaccines

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...