卵巢刺激对人类子宫内膜微环境的影响
Anat Chemerinski1, May Shen2, Nuriban Valero-Pacheco3
1Department of Obstetrics, Gynecology and Reproductive Health, Rutgers Biomedical and Health Sciences, Newark, NJ, USA.
Human reproduction (Oxford, England)
|March 21, 2024
概括
卵巢刺激 (OS) 显著改变了周排卵性子宫内膜.
科学领域:
- 生殖内分泌学和不孕不育问题
- 人类胚胎学与发育研究
- 生殖免疫学 生殖免疫学
背景情况:
- 卵巢刺激 (OS) 对于辅助生殖技术 (ART) 至关重要,但可能会影响子宫内膜受容性.
- 以前的研究表明,OS改变子宫内膜基因表达和免疫细胞群,可能影响怀孕结果.
- 在周排卵期和中分泌期期间,OS对子宫内膜的腺体,肌层,免疫和血管区的具体影响尚不清楚.
研究的目的:
- 调查卵巢刺激 (OS) 如何影响人类子宫内膜的细胞和转录基因特征.
- 为了比较这些效应在周排卵期相对中分泌期 (植入窗口).
- 阐明OS对子宫内膜腺体,肌层,免疫和血管区的影响.
主要方法:
- 前性队列研究比较了25名接受OS的受试者与25名自然月经周期的受试者.
- 在增殖,周排卵和中分泌阶段收集的子宫内膜活检.
- 分析包括子宫内膜约会,基因和蛋白质表达,细胞群体分析和3D结构评估;血清激素水平也被测量.
主要成果:
- 在产卵期期间,OS诱导了显著的转录基因变化,腺体-流体失调,增加腺体上皮体体积,以及更高的子宫内膜单细胞/巨细胞的频率.
- 在中间分泌阶段 (植入窗口),OS主要改变了免疫细胞群,增加了B细胞和减少了CD4效应T细胞.
- 与中分泌期子宫内膜相比,在OS下,周排卵期子宫内膜显示出更实质的转录和细胞变化.
结论:
- 卵巢刺激显著影响周周排卵期子宫内膜的转录组和细胞组成.
- 虽然OS也会影响植入窗口中的免疫细胞,但子宫内膜环境与自然周期的相似性比较多.
- 在OS下对子宫内膜免疫区的功能进行进一步的研究是有必要的,以了解其对ART成功的影响.
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