阿迪波涅克受体激进二Tyr-Pro刺激NE-4C细胞中的乙胆神经系统
Lihong Cheng1, Caiyue Shi1, Saya Nakamura1
1Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.
Journal of agricultural and food chemistry
|March 21, 2024
概括
滴Tyr-Pro通过激活神经细胞中的阿迪波涅丁受体1 (AdipoR1) 和胆乙转移酶 (ChAT) 来刺激乙胆 (ACh) 的产生,这表明潜在的认知健康.
科学领域:
- 神经科学是一个神经科学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 双Tyr-Pro具有大脑运输,预防认知障碍和阿迪波涅克丁受体1 (AdipoR1) 激动作用的潜力.
- 了解Tyr-Pro对乙胆 (ACh) 神经系统的影响对于神经退行性疾病研究至关重要.
研究的目的:
- 为了研究双Tyr-Pro对NE-4C神经细胞内的乙胆 (ACh) 神经系统的影响.
- 阐明Tyr-Pro对ACh生产和相关途径的作用的潜在分子机制.
主要方法:
- 用Tyr-Pro和AdipoRon (AdipoR1激动剂) 刺激NE-4C神经细胞,以测量ACh的产生.
- 对AdipoR1和胆酸转移酶 (ChAT) 表达的分析.
- AdipoR1敲击实验以评估Tyr-Pro的机制,包括AMPK和ERK1/2激活.
主要成果:
- Tyr-Pro 和 AdipoRon 诱导了度依赖的 ACh 生产,并在 NE-4C 细胞中增加了 AdipoR1 和 ChAT 表达.
- 在AdipoR1被淘汰后,Tyr-Pro促进了ChAT表达,并激活了AMPK和ERK1/2通路.
- Tyr-Pro没有影响乙胆酶或ACH受体,这表明它可以作为ACH加速剂发挥作用.
结论:
- Tyr-Pro作为阿迪波R1激动剂,通过阿迪波R1诱导的CHAT激活刺激ACh神经系统.
- 这项研究提供了Tyr-Pro在通过AdipoR1信号传递增强ACh神经递质作用的第一个证据,突出了其潜在的治疗价值.
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