广泛的宿主范围的菌体向全球Clostridium perfringens的细菌菌株,并在五种菌株模型系统中清晰感染
Anisha M Thanki1, Emmanuel K Osei2,3, Natasha Whenham4
1Department of Genetics and Genome Biology, Leicester Centre for Phage Research, University of Leicester, Leicester, United Kingdom.
Microbiology spectrum
|March 21, 2024
概括
菌体 (菌体) 在治疗由Clostridium perfringens引起的家禽感染方面表现有前途. 一种特定的菌体尾酒有效地减少了C. perfringens在幼虫模型中的殖民,这表明了预防和治疗感染的潜力.
科学领域:
- 兽医微生物学 兽医微生物学
- 细菌治疗疗法是一种细菌治疗.
- 禽类的健康 禽类的健康
背景情况:
- 杆菌是家禽中主要的细菌病原体,导致死肠炎和显著的死亡率.
- 在C. perfringens中抗菌素耐药性需要替代治疗,与家禽行业减少抗生素使用的目标保持一致.
- 菌体 (菌体) 是感染和杀死细菌的病毒,为抗生素提供了潜在的治疗替代品.
研究的目的:
- 为了隔离和描述对全球传播的Clostridium perfringens菌株有效的细菌菌体.
- 评估单个菌体和菌体尾酒在减少C. perfringens殖民的有效性.
- 建立和利用C. perfringens幼虫感染模型,模仿家禽感染,用于菌体疗效测试.
主要方法:
- 隔离和表征32种向C. perfringens的细菌菌体.
- 对97个全球家禽进行宿主范围查C. perfringens分离物.
- 在液体培养和多菌株C. perfringens幼虫感染模型中进行病毒性测试.
- 在幼虫模型中对16种菌体尾酒的有效性测试.
主要成果:
- 在32个分离的菌体中,有19个化了全球C. perfringens家禽菌株集合的80%-92%.
- 包含CPLM2,CPLM15和CPLS41的菌体尾酒显示出最高的疗效.
- 选择的菌体尾酒在幼虫感染模型中主动施用时,显著减少了C. perfringens的殖民.
结论:
- 菌体是控制家禽中Clostridium perfringens的一种可行的治疗选择.
- 菌体尾酒,特别是CPLM2,CPLM15和CPLS41组合,显示出预防和治疗C. perfringens感染的巨大潜力.
- 开发的幼虫感染模型有效模拟家禽感染,并可用于评估菌体的疗效.
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