[肉抗人类B7-H4 IgY多克隆抗体的制备和特征]
Li Zhang1, Zhenjun Fan1, Wenting Zhang1
1Institute of Laboratory Medicine, College of Medical Technology, Guangdong Medical University, Dongguan 523808, China.
概括
研究人员开发了一种针对人类B7-H4 (B7同源4) 的高特异性抗体,以及一种敏感的ELISA测试,用于检测血清中可溶性B7-H4蛋白,有助于卵巢癌诊断.
科学领域:
- 免疫学 免疫学 免疫学
- 生物技术是生物技术.
- 在瘤学瘤学.
背景情况:
- B7同源4 (B7-H4) 是一种免疫检查点分子,与癌症进展有关.
- 在血清中检测可溶B7-H4 (sB7-H4) 对癌症诊断和监测有价值.
- 现有的检测方法可能缺乏足够的灵敏度或特异性.
研究的目的:
- 为了产生一种的抗人类B7-H4卵黄免疫球蛋白 (IgY) 多克隆抗体.
- 建立一个敏感和特定的双抗体三明治ELISA,用于在人血清中检测sB7-H4.
主要方法:
- 生物信息学被用来识别人类sB7-H4.4的特定B细胞表位.
- 产母被免疫,用来产生抗B7-H4IgY抗体,这些抗体被净化.
- 抗体表征涉及ELISA,西部斑点和流细胞计.
- 开发了一种双抗体三明治ELISA,并使用临床样本与商业套件进行验证.
主要成果:
- 成功制备了高度特异的抗人类B7-H4 IgY多克隆抗体.
- 开发的ELISA检测出卵巢癌和良性瘤患者的sB7-H4水平明显升高,而不是健康的对照.
- 与商业ELISA套件相比,基于IgY的ELISA显示出更高的灵敏度,结果一致.
结论:
- 可以有效地产生抗人类B7-H4IgY多克隆抗体.
- 成功建立了一个强大的双抗体三明治ELISA,用于检测人类血清中的sB7-H4.
- 这种测试为卵巢癌诊断中的潜在应用提供了一个敏感的工具.
相关概念视频
01:10Antibody Structure
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:31Hybridoma Technology
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...