代谢学方法探索来自植物根相关的链状菌株的生物活性天然产品
1The Institute of Natural and Applied Science, Dicle University, Diyarbakır, Turkey.
Applied biochemistry and biotechnology
|March 21, 2024
概括
植物根Streptomyces细菌产生各种生物活性代谢物,具有广泛的抗菌活性. 这项研究强调了它们在发现新型抗生素和用于制药应用的天然产品方面的潜力.
科学领域:
- 微生物学 微生物学
- 自然产品化学 自然产品化学
- 代谢学 代谢学 代谢学
背景情况:
- 细菌Streptomyces是临床抗生素的主要来源.
- 与植物相关的Streptomyces菌株具有生产生物活性天然产品的尚未开发的潜力.
- 这些天然产品可以增强植物对病原体的防御能力.
研究的目的:
- 从植物根中分离出来的Streptomyces菌株中选生物活性代谢物.
- 研究不同培养条件下产生的代谢物的化学多样性.
- 评估这些代谢物的抗菌活性.
主要方法:
- 使用一株多化合物 (OSMAC) 方法进行培养.
- 雇佣液体染色学-并行质谱学 (LC-MS/MS) 和全球天然产品社会分子网络 (GNPS MN) 进行代谢物分析.
- 使用磁盘扩散方法评估抗菌活性.
主要成果:
- 所有测试的Streptomyces菌株都表现出广泛的抗菌活性.
- 代谢物概况根据培养介质和溶剂提取有显著的变化.
- 来自Streptomyces sp. 的提取物. CAH29显示出对黄金葡萄球菌和白菌的强烈抑制.
- 识别了已知的抗生素 (spiramycin,actinomycin),一个瘤促进剂 (lyngbatoxin) 和许多未知的化合物.
结论:
- 来自植物根的Streptomyces菌株是各种生物活性二次代谢物的宝贵来源.
- 包括GNPS MN在内的代谢学方法是快速选生物活性代谢物产生微生物的有效工具.
- 新型化合物的发现表明了新的药物开发的潜力.
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