相关实验视频

Updated: Jun 30, 2025

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

基于氨基酸代谢相关基因的骨肉瘤的预后签名

Liwen Feng^1,2, Yuting Chen², Xiangping Mei²

¹Department of Oncology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Cancer biotherapy & radiopharmaceuticals

|March 21, 2024

概括

这项研究确定了四种氨基酸代谢相关基因 (MAG),这些基因可以预测骨髓瘤 (OS) 的存活率. 一个基于MAG的新型签名为开发新的OS治疗提供了潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

骨髓瘤 (OS) 是一种具有挑战性的骨癌,治疗选择有限.
改变的氨基酸 (AA) 代谢与OS发展有关.
识别关键的代谢相关基因 (MAG) 对于理解OS进展至关重要.

研究的目的:

在骨髓瘤 (OS) 中识别差异表达的代谢相关基因 (MAG).
根据MAGs开发OS的预后风险签名.
探索这些MAG的生物功能和临床影响.

主要方法:

利用了来自GEO和UCSC Xena数据库的基因表达特征和临床数据.
使用生物信息学分析 (limma,Cox回归) 识别了差异表达基因 (DEG) 和预后MAG.
验证了基于MAG的签名,并构建了一个用于生存预测的诺米克图;通过RT-qPCR,西斑和IHC确认了基因表达.

主要成果:

确定了790个DEG和62个预后性MAG.
开发了一个四个基因的MAG签名 (PIPOX,PSMC2,SMOX,PSAT1) 与验证的预后值 (AUCs~0.71-0.72).
这种特征与自然杀手细胞透和抗血管原体耐药性相关;名图显示出良好的预测准确性 (C指数为0.77).

结论:

四个氨基酸代谢相关基因 (MAG) 是骨髓瘤患者生存的预后.
开发的基于MAG的签名显示了指导OS治疗策略的潜力.
这种签名可能为骨髓瘤的发病和治疗发展提供有价值的见解.

关键词:

地理地质组织 (GEO)在UCSC的Xena.氨基酸代谢氨基酸代谢生物信息学是一种生物信息学.骨的瘤骨的瘤预测模型预测模型

更多相关视频

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Evaluation of Amino Acid Consumption in Cultured Bone Cells and Isolated Bone Shafts

Published on: April 13, 2022

相关概念视频

mTOR Signaling and Cancer Progression

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...