普拉诺科库斯·诺塔坎蒂 (Planococcus notacanthi) sp. sp. 没有被发现. Nov.,从深海鼻的皮肤上分离出来
Shona Uniacke-Lowe1,2,3, Catherine Stanton2,3, Colin Hill1,2
1School of Microbiology, University College Cork, Cork, Ireland.
概括
一种新的细菌,Planococcus notacanthi,在深海上被发现. 这种有氧,运动性的菌体表现出抗菌性质,并代表了Planococcus属中的新物种.
科学领域:
- 微生物学 微生物学
- 海洋生物学 海洋生物学
- 细菌学 细菌学是一门学科.
背景情况:
- 一种新的细菌菌株,APC 4016T,从从北大西洋深处收集的鼻,Notacanthus chemnitzii的皮肤中分离出来.
- 隔离发生在1000米深处,突出显示了细菌起源的独特环境.
研究的目的:
- 为了描述新型细菌菌株APC 4016T.
- 为了确定其在Planococcus属中的分类地位.
- 根据遗传学和表型数据提出一种新的物种名称.
主要方法:
- 表型特征包括细胞形态,格拉姆染色,运动性和生长条件 (温度,pH,盐度).
- 16S rRNA基因测序用于遗传学分析.
- 数字DNA-DNA杂交 (dDDH) 和平均核酸标识 (ANI) 计算用于基因组比较.
- 对细胞脂肪酸,呼吸和极性脂质的分析.
- 基因组测序以确定基因组大小草案和G+C含量.
主要成果:
- 菌株APC 4016T是有氧的,运动的,Gram-阳性到Gram-变量,并形成色色素的殖民地.
- 在25-28°C,pH值7-7.5和1%NaCl的环境下生长最佳.
- 遗传学分析将其置于Planococcus属内,与Planococcus okeanokoites密切相关 (98.98%的16S相似性).
- 低的dDDH (20.1%) 和ANI (83.8%) 值使其与P.区别开来. 海洋科科特 (Oceanokoite) 是一种海洋科特.
- 鉴定出了不同的细胞脂肪酸,呼吸和极性脂质配置文件.
- 基因组草案是3.6 Mb,含有45.25%的G + C内容.
- 该菌株具有抗微生物活性和细菌素和二次代谢物生产的基因集群.
结论:
- 菌株APC 4016T代表了Planococcus属中的一种新物种.
- 这种新物种的拟议名称是Planococcus notacanthi sp. 在新的一年里.
- 类型菌株是APC 4016T (=DSM 115753T=NCIMB 15463T) 的.
- 这一发现有助于了解深海环境中的微生物多样性以及新生物活性化合物的潜力.
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