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在Drosophila melanogaster丸中,mTORC1对于生殖系干细胞的分化是必需的

Marie Clémot^1,2, Cecilia D'Alterio¹, Alexa C Kwang¹

¹Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, United States of America.

|March 21, 2024

概括

拉巴胺素的目标 (TOR) 途径调节新陈代谢和干细胞分化. 在多索菲拉雄性生殖系干细胞 (GSCs) 中降低mTOR的调节阻断了分化并破坏了组织平衡.

科学领域:

细胞生物学细胞生物学
发展生物学发展生物学
代谢研究研究的代谢研究.

背景情况:

代谢对于干细胞功能和组织平衡至关重要.
拉巴胺素的目标 (TOR) 途径调节新陈代谢和干细胞的维护/分化.
在与信号线索协调的成年干细胞中TOR的确切作用尚不清楚.

研究的目的:

研究TOR途径在调节男性生殖系干细胞 (GSC) 分化中的作用.
了解mTORC1在GSC中的活性如何影响Drosophila的分化和组织稳态.

主要方法:

利用针对性RNAi介导的mTor在Drosophila雄性生殖系干细胞和早期生殖细胞中的下调.
观察到对生殖细胞分化,GSC类特征和自溶酶体形态学的影响.
评估了对邻近囊细胞的非自主影响以及生殖系/体质分化的协调.

主要成果:

早期生殖细胞中mTor的下调导致分化阻断,积累了类似GSC的细胞.
这些细胞表现出大型的,功能失调的自溶酶体.
减少的mTor导致囊细胞中的mTORC1非自主激活,破坏了生殖线-体质分化协调.

结论:

在男性生殖线分化中,TOR途径起着至关重要的,以前未被描述的作用.
在生殖系干细胞中mTORC1信号传递对于协调生殖系和体的分化至关重要.
对TOR信号的调节失调会影响干细胞功能和组织恒温.

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