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Updated: Jun 30, 2025

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
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A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

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转基因转移酶聚基酶的进化指导工程

Mathijs F J Mabesoone1, Stefan Leopold-Messer1, Hannah A Minas1

  • 1Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, Vladimir-Prelog-Weg 4, 8093 Zürich, Switzerland.

Science (New York, N.Y.)
|March 21, 2024
PubMed
概括

工程细菌聚基合成酶 (PKSs) 是发现新药的关键. 这项研究确定了转转移酶 (trans-AT) PKS 中的一个常见模块位点,用于成功的酶工程,从而能够创建新型的多基结构.

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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

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相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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