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识别新型比萨米德装饰的松衍生物作为抗植物病原病毒剂:生物活性评估和计算模拟

Zhen-Xing Li¹, Yue Ding¹, Tai-Hong Zhang¹

¹National Key Laboratory of Green Pesticide, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Center for R&D of Fine Chemicals of Guizhou University, Guiyang 550025, China.

Journal of agricultural and food chemistry

|March 21, 2024

概括

新型比萨米德装饰的黄衍生物对烟草马赛克病毒 (TMV) 具有强大的抗病毒活性. 化合物7d有效地抑制TMV组装和基因表达,为农业提供了一个有前途的新型杀毒候选者.

科学领域:

农业科学农业科学
有机化学有机化学
分子生物学分子生物学

背景情况:

烟草马赛克病毒 (TMV) 在全球显著影响作物产量和质量.
开发有效的病毒杀菌剂对于农业的可持续性至关重要.
装饰着比萨米德的佐特醇衍生物代表了一种新型的潜在抗病毒药物.

研究的目的:

合成和选针对TMV的抗病毒活性新 bisamide 装饰的佐特醇衍生物.
为了评估与商业抗病毒剂相比,化合物7d的疗效.
阐明化合物7d.的初步抗病毒作用机制.

主要方法:

佐特醇衍生物的化学合成和生物选.
使用EC50测定进行抗病毒活性测试.
作用研究的机制包括传输电子显微镜,MST,RT-qPCR和西方涂料.
用于绑定分析的计算模拟.

主要成果:

与瑞巴维林 (EC50 = 442.1 μg/mL) 相比,化合物7d对TMV表现出更高的抗病毒活性 (EC50 = 157.6 μg/mL).
发现化合物7d通过与外衣蛋白结合 (Kd = 0.7 μM) 来抑制TMV组合.
它还抑制了TMV外套蛋白的基因表达和生物合成,计算预测了强大的结合相互作用.

结论:

装饰在比萨米德的佐特醇衍生物具有显著的病毒抑制潜力.
化合物7d是农业化学应用中非常有前途的杀毒候选物.
这些化合物的进一步开发可能会导致新的策略来管理作物中的TMV感染.

关键词:

TMVV TMVV 在线观看抗病毒特征抗病毒特征班佐特里亚佐尔 (benzotriazole) 是一种可以毛皮蛋白质是一种毛皮蛋白质.计算模拟的计算模拟.

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