相关实验视频
Updated: Jun 30, 2025

07:01
Manipulation of Gene Function in Mexican Cavefish
Published on: April 22, 2019
9.1K
安第斯山洞鱼Trichomycterus rosablanca (Siluriformes,Trichomycteridae) 的参考基因组:建立基因组资源,研究洞穴环境中的进化
Carlos Daniel Cadena1, Laura Pabón1, Carlos DoNascimiento2
1Departamento de Ciencias Biológicas, Universidad de los Andes, Bogotá, Colombia.
The Journal of heredity
|March 21, 2024
概括
研究人员对盲目鱼Trichomycterus rosablanca的基因组进行了测序,这是研究洞穴动物融合进化的关键一步. 这种基因组资源有助于理解形态学的重复进化变化.
科学领域:
- 进化生物学 进化生物学
- 基因组学就是基因组学.
- 规格 规格 规格 规格
背景情况:
- 居住在洞穴中的动物为融合进化提供了洞察力.
- 哥伦比亚的Trichomycterus鱼表现出对洞穴的独立适应,失去眼睛和色素.
- 基因组研究对于理解重复形态进化的机制至关重要.
研究的目的:
- 为了建立Trichomycterus rosablanca的高质量参考基因组.
- 为研究洞穴鱼融合进化的基因组基础提供基础资源.
- 支持对驱动Trichomycterus表型变化的进化力量的研究.
主要方法:
- 一个双倍体,染色体尺度,长读参考基因组的生成.
- 使用了HiFi长读测序技术.
- 使用N50统计和BUSCO (基因组组装和质量评估) 的基因组组装和质量评估.
主要成果:
- 在27个染色体中组装了一个1Gb的核基因组.
- 实现了 40.0× HiFi 长读覆盖.
- 高连接性与N50脚手架的40.4 Mb和N50连接的13.1 Mb.
- 组装证明了高完整性,其中96.9%的Eukaryota和95.4%的Actinopterygii BUSCOs.
结论:
- 罗萨布兰卡菌 (Trichomycterus rosablanca) 的基因组组合是该属的第一个.
- 这种参考基因组是进化和基因组研究的重要资源.
- 促进对洞穴环境中融合进化和适应的遗传基础的研究.
相关概念视频
Evolutionary Relationships through Genome Comparisons
5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K
Gene Evolution - Fast or Slow?
7.1K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.1K
Cis-regulatory Sequences
9.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.9K
Convergent Evolution
27.7K
Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
27.7K
Multi-species Conserved Sequences
3.9K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.9K
Genetic Screens
4.9K
Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which...
4.9K

