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Huan Wang¹, Yue Jia¹, Tao Feng¹

¹Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, University of Jinan, Jinan, 250022, China.

|March 21, 2024

概括

一个新的DNA感应微芯片检测到的吐毒素 (deoxynivalenol,DON). 这种可重复使用的生物传感器使用量子点和放大电化学发光信号来进行敏感的DON检测.

科学领域:

分析化学分析化学
材料科学材料科学材料科学
生物技术是生物技术.

背景情况:

嘔吐毒素 (deoxynivalenol, DON) 是一種菌毒素,會污染食物作物,如,造成健康風險.
准确而敏感的DON检测方法对于食品安全和农业监测至关重要.
现有的检测方法可能缺乏可重复使用性,敏感性或需要复杂的样本准备.

研究的目的:

开发一种可重复使用的DNA传感微芯片,用于在中敏感检测脱氧尼瓦伦醇 (DON).
使用基于的核心外量子点 (CdSe@CdS QDs) 作为用于DON检测的电化学发光 (ECL) 发射器.
为了增强ECL信号,使用金纳米粒子修改加速器来提高灵敏度.

主要方法:

准备大小可调的水相CdSe@CdS QDs,具有强烈的正极ECL发射.
用金纳米颗粒修改的铁化水合物 (Fe-Co-Au) 作为加速器对微芯片的修改.
开发一种基于DNA的传感机制,其中DON结合会通过aptamer-DNA相互作用和铁介导电子转移触发信号生成.

主要成果:

开发的微芯片证明了在中敏感地检测到DON.
通过Fe-Co-Au加速器有效地放大了ECL信号.
传感机制依赖于一个火器 (ssDNA S2) 在DON与阿普坦酶 (ssDNA S1) 结合时的位移.
确定了DON的检测范围在1 ng/mL和200 ng/mL之间.

结论:

基于CdSe@CdS QDs和ECL的可重复使用的DNA传感微芯片已成功开发用于DON检测.
该传感器提供了一种可行和灵敏的方法,用于在样品中量化DON.
这种方法有可能在农产品中进行快速和可靠的真菌毒素监测.

关键词:

生物感知微芯片的使用在CdSe@CdS QDs中使用.电化学的发光产生了光.嘔吐毒素 (Vomitoxin) 是一種吐毒素.

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