阿拉比多普西斯核糖体RNA处理鱼突变体表现出悬浮体衍生的多胚胎性,这是由于悬浮体的直接重编程
Honglei Wang1, Luca Santuari2, Tristan Wijsman1
1Cluster of Plant Developmental Biology, Laboratory of Cell and Developmental Biology, Wageningen University & Research, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
The Plant cell
|March 21, 2024
概括
阿拉比多普西斯UTP18的突变导致悬浮体衍生的多胚胎,延迟了胚胎的正常发育. 这突显了非细胞自主信号,防止悬浮体重编程到胚胎中.
科学领域:
- 植物发育生物学植物发育生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 阿拉比多普西斯胚胎的发育始于不对称的胚胎分裂,形成胚胎本身和悬浮体.
- 悬浮剂通常在心脏阶段后退化,但在早期保留了胚胎潜力.
- 了解控制悬浮器命运的因素对于发育可塑性至关重要.
研究的目的:
- 调查Arabidopsis.在悬浮剂衍生的多胚胎的遗传基础.
- 为了识别对-1 (mrl-1) 突变表型负责的基因.
- 阐明UTP18在早期胚胎发生和悬浮体发育中的作用.
主要方法:
- 显示多胚胎的mrl-1突变体的特征.
- 在UTP18基因中对因果突变的遗传映射和鉴定.
- 通过淘汰突变物和分子分析分析UTP18功能的分析.
- 研究涉及悬浮体命运决定的信号通路.
主要成果:
- mrl-1突变是UTP18促进体中的基因组重组,导致高透度悬浮剂衍生的多胚胎.
- 在胚胎正确发育初期减少的UTP18蛋白水平引发了这种现象.
- 根特异性UTP18淘汰会导致生长停止和未经处理的18S前rRNA积累.
- 一种功能丧失的突变体 (mrl-2) 显示出异步的大甲基形发育和胚胎囊堕胎.
结论:
- 在早期胚胎正确发育期间,UTP18丰富度的降低启动了悬浮体衍生胚胎生成.
- 这些发现支持胚胎本身存在非细胞自主信号,抑制悬浮体重编程.
- UTP18在调节正常阿拉比多普西斯胚胎发生过程中必不可少的rRNA处理方面发挥着关键作用.
相关概念视频
Riboswitches
8.1K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
8.1K
Ribosomal RNA Synthesis
13.2K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.2K
Pre-mRNA Processing: Modification of pre-mRNA Ends
9.3K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
9.3K
pre-mRNA Processing
52.9K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
52.9K
Pre-mRNA Processing: RNA Splicing
5.2K
5.2K
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K


