由植物MLKLs介导的细胞质流入赋予了TNL触发的免疫力
Qiaochu Shen1, Keiichi Hasegawa2, Nicole Oelerich3
1Institute for Plant Sciences, University of Cologne, 50674 Cologne, NRW, Germany.
Cell host & microbe
|March 21, 2024
概括
植物混血基因酶域样 (MLKL) 蛋白质对于托尔-互乐金-1受体域NLR (TNL) 触发的免疫非常重要. 阿拉比多普西斯MLKL1聚合在血膜上,并调解的流入,揭示了并行的免疫信号通路.
科学领域:
- 植物免疫力 植物免疫力
- 蜂信号传输是如何进行的
- 分子生物学分子生物学
背景情况:
- 植物混合系基因酶域样 (MLKL) 蛋白质是Toll-interleukin-1受体域NLR (TNL) 受体触发免疫反应的关键组成部分.
- 脊椎动物中的MLKL同类物已知诱导了亡,这是一个被编程的细胞死亡途径.
研究的目的:
- 调查阿拉比多普西斯MLKL1 (AtMLKL1) 在TNL触发的免疫力中的作用.
- 阐明AtMLKL1功能背后的分子机制,包括其亚细胞局部化和信号传递能力.
主要方法:
- 同焦显微镜观察AtMLKL1在等离子膜上的聚合.
- 基因分析涉及TNL信号传感器,如EDS1.1.
- 功能性试验评估AtMLKL1在免疫和 ([Ca2+]cyt) 流入中的作用.
- 在植物和人类细胞中的比较研究.
主要成果:
- 在TNL激活时,Arabidopsis MLKL1 (AtMLKL1) 在血膜上形成点点,依赖于EDS1.1.
- 在TNL触发的免疫中,AtMLKLs与RPW8型含有Helo域的NLRs (RNLs) 并行运作.
- AtMLKL的N端HeLo域对于免疫和集群都至关重要.
- 在AtMLKLHeLo域中介于植物和人类细胞中的细胞质Ca2+ ([Ca2+]cyt) 流入.
- 在TNL引发的,但不是CNL引发的免疫过程中,AtMLKLs负责持续的[Ca2+]细胞流入.
结论:
- 植物MLKL和RLN表现出并行的免疫信号功能.
- 在植物免疫反应期间,MLKLs作为细胞质Ca2+ ([Ca2+]cyt) 流入的调解者.
- HeLo 域可能代表了 Ca2+ 信号中继在多种生物体中保存的结构图案.
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