相关实验视频

Updated: Aug 1, 2026

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Published on: June 29, 2011

微囊 (Microcystis aeruginosa) 的β-cyclocitral诱导的细胞迅速死亡

Xuejian Wang¹, Huansheng Cao², Yinjie Zhu¹

¹Groundwater Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong, 518055, China; Key Laboratory of Microorganism Application and Risk Control (MARC) of Shenzhen, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, Guangdong, 518055, China.

Environmental pollution (Barking, Essex : 1987)

|March 21, 2024

概括

β-环 (BCC) 通过破坏细胞结构和抑制光合作用,导致蓝菌细胞死亡. 这项研究揭示了BCC.

科学领域:

环境微生物学环境微生物学
蓝藻细菌生物学蓝藻细菌生物学
生物化学毒理学生物化学毒理学

背景情况:

像Microcystis aeruginosa这样的开花形成的蓝藻细菌产生β-环素 (BCC).
假设BCC是由于其溶解效应导致蓝藻细菌花的下降.
在蓝藻细菌上BCC的致命性背后的机制在很大程度上仍未被探索.

研究的目的:

研究Microcystis aeruginosa对β-cyclocitral (BCC) 的细胞,分子和转录基因反应.
阐明蓝藻细菌中BCC诱导的细胞死亡的机制.
评估BCC作为一种有害藻类繁殖控制工具的潜力.

主要方法:

暴露于不同度的BCC (0-300毫克/升) 的M. aeruginosa.
使用扫描电子显微镜 (SEM) 进行细胞分析.
生理学测量 (光合作用活动,叶绿素a,ATP,谷氨).
压力和细胞死亡相关基因的转录基因分析.

主要成果:

BCC表现出度依赖的致命效应,在150-300 mg/L时引起细胞破裂.
SEM揭示了形态变化,包括收缩和细胞穿孔.
BCC抑制了光合作用活性,降低了甲和ATP含量,并破坏了氧化还原稳定.
转录基因数据表明SOS反应和mazEF系统基因的上调,这表明类似于亡和mazEF介导的死亡途径.

结论:

通过不同的途径,BCC诱导了蓝藻细菌的编程细胞死亡.
BCC的溶解和生理效应有助于蓝藻细菌的花死亡.
BCC显示了控制有害藻类繁殖的潜力.

关键词:

蓝藻细菌是一种蓝藻细菌.抑制生长抑制生长抑制氧化应激是一种氧化应激.光合作用的损伤.调节的细胞死亡.

更多相关视频

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Published on: January 29, 2016

Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

Metabolic Profiling to Determine Bactericidal or Bacteriostatic Effects of New Natural Products using Isothermal Microcalorimetry

Published on: October 29, 2020

相关概念视频

Cell Motility through Blebbing

Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...

Bacterial Phylum Cyanobacteria

Bacterial Phylum Cyanobacteria

Cyanobacteria are a diverse group of oxygenic, phototrophic bacteria that played a pivotal role in converting Earth’s atmosphere from anoxic to oxygen-rich billions of years ago. They exhibit remarkable morphological diversity, ranging from unicellular forms to filamentous types, with cell sizes varying between 0.5 μm and 100 μm. Cyanobacteria are classified into five groups: Chroococcales (unicellular, dividing by binary fission), Pleurocapsales (unicellular, dividing by multiple fission),...