对Helicobacter pylori和宿主细胞转录组的双RNA测序揭示了本体学上不同的宿主-病原体相互作用
Wei Hu1,2, Zhi Yong Zhai1,2, Zhao Yu Huang1,2
1Department of Gastroenterology, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong, China.
mSystems
|March 22, 2024
概括
杆菌感染会改变宿主细胞的过程和细菌的基因表达. 这项研究揭示了H. pylori.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 宿主-病原体相互作用
背景情况:
- 杆菌是人类重要的病原体.
- 螺杆菌和胃上皮之间的相互作用是复杂的,并未完全理解.
- 细胞毒素相关基因A (cagA) 在H. pylori病变发生过程中起着关键作用.
研究的目的:
- 通过使用双RNA测序来研究H. pylori和人类胃上皮细胞之间的动态相互作用.
- 描述由cagA调节的H. pylori适应策略.
- 探索H. pylori感染对宿主细胞过程的影响及其对大肠炎的保护作用.
主要方法:
- 与人类胃上皮细胞共同培养的H. pylori的双RNA测序.
- 细菌基因表达的分析,专注于ATP结合的磁带载体和电子传输基因.
- 宿主mRNA的时间分析,以确定拼接调节器和细胞周期基因的变化.
- 在体外和体内实验中评估H. pylori殖民对DSS诱导的大肠炎的影响.
主要成果:
- 通过调节metQ和HP_0888的表达和抑制电子运输基因,激活氧化酸化,CagA调节细菌的适应.
- 杆菌感染导致宿主拼接调节者的下调,导致细胞周期相关基因中异常的mRNA前拼接.
- 胃H. pylori的殖民化可以防止慢性DSS诱导的大肠炎.
- 在H. pylori殖民的小鼠中,富含和黄油酸生产细菌 (Muribaculaceae) 促进了肠道屏障的修复.
结论:
- 这项研究提供了首次对H. pylori-胃上皮细胞相互作用的双转录组分析.
- 对H. pylori的适应策略和胃上皮质病原机制的新见解.
- H. pylori殖民可能通过调节肠道微生物群来提供对肠道炎症的保护作用.
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