一个免疫类型结合的转录基因地图书人类的血型造血祖先
Xuan Zhang1, Baobao Song1,2, Maximillian J Carlino3,4
1Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Nature immunology
|March 22, 2024
概括
人类骨髓的单细胞CITE-seq分析显示了80多种细胞类型. 这本先进的地图集识别了细胞表面标记物和急性髓性白血病干细胞的正常等价物.
科学领域:
- 血液学 血液学 血液学
- 免疫学 免疫学 免疫学
- 基因组学就是基因组学.
- 单细胞生物学 单细胞生物学
背景情况:
- 单细胞多模式方法正在彻底改变人类造血原始体的分析.
- 通过测序 (CITE-seq) 来对转录体和表观体进行细胞索引,可以同时对表面蛋白和转录体进行分析.
研究的目的:
- 系统地对初级人类骨髓细胞进行CITE-seq.
- 优化抗体面板,用于全面的细胞表面标记物和转录组分析.
- 为了创建一个详细的血造原生细胞和它们的状态的地图.
主要方法:
- 使用了266个CITE-seq抗体和机器学习来选择132个抗体的优化面板.
- 进行多式分析,以基于表面标记物和转录组来解决不同的细胞群.
- 调整注释以指定特定急性髓性白血病干细胞种群的正常骨髓等价物.
主要成果:
- 解析了80多种不同的细胞类型,包括干细胞,原生细胞,免疫细胞,树皮细胞和过渡细胞.
- 识别了许多细胞表面标记物,在各种捐赠者 (跨越种族和性别) 中始终检测到.
- 启用流式细胞测量解决方案用于in silico预测的细胞状态.
结论:
- 开发的地图集为分析健康和疾病中的人类造血原生细胞提供了全面的资源.
- 这些发现有助于识别急性髓性白血病干细胞的正常造血对应物,并可能为临床反应分层提供信息.
- 这项工作促进了对骨髓微环境中的细胞异质性的理解.
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