小型原氨基酸用于基导向的无背景成像

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Updated: Jun 17, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

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Fabio de Moliner1, Zuzanna Konieczna2, Lorena Mendive-Tapia1

  • 1Centre for Inflammation Research The University of Edinburgh UK.

Angewandte Chemie (Weinheim an der Bergstrasse, Germany)
|March 22, 2024
PubMed
概括

研究人员为超分辨率显微镜开发了新的光氨基酸. 这些二醇记者使的背景图像能够免费成像,改善生物样本的可视化.

关键词:
光是一种光效应.显微镜的使用方法探测器 探测器蛋白质 蛋白质 蛋白质超级分辨率的超级分辨率

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...