滑动运动性蛋白质GldJ和SprB有助于Flavobacterium columnare的毒性
Nicole C Thunes1, Jason P Evenhuis2, Ryan S Lipscomb2
1Department of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin, USA.
Journal of bacteriology
|March 22, 2024
概括
弗拉沃细菌 (Flavobacterium columnare) 的滑动性有助于杆菌疾病的毒性. 移动性有缺陷的突变者表现出减少的毒性,幸存者获得了对未来感染的部分抵抗力.
科学领域:
- 微生物学 微生物学
- 鱼类病理学 鱼类病理学
- 细菌的毒性因素 细菌的毒性因素
背景情况:
- Flavobacterium columnare会导致columnaris病,这是水产养殖的重大威胁.
- 第九类分泌系统 (T9SS) 对F. columnare的运动性和毒性至关重要,但运动性的具体作用尚不清楚.
- 由于对疾病机制的不完全理解,现有的对立柱病的控制措施不足.
研究的目的:
- 为了阐明F. columnare滑动运动在columnaris疾病的发病过程中的作用.
- 区分运动性和T9SS介导分泌对细菌毒性的贡献.
- 调查新型疾病控制战略的潜在途径.
主要方法:
- 构建和分析具有运动缺陷的F. columnare突变体 (sprB,sprF,gldJ删除突变体).
- 产生一个gldJ截断突变体 (gldJ563) 来分离运动和分泌功能.
- 通过彩虹鱼幼的挑战测试来评估细菌毒性.
- 对鱼类的获得性耐药性的评估,这些鱼类在暴露于突变菌株后幸存下来.
主要成果:
- 在滑翔机动性 (sprB,sprF,gldJ删除) 缺陷的突变体在彩虹鱼中表现出减少的毒性.
- 一种gldJ截断突变 (gldJ563),在滑翔中受损,但保留分泌能力,也显示出病毒毒性降低,强烈表明运动性对病毒毒性有所贡献.
- 活着暴露在运动缺陷突变的鱼类对随后的野生类型F.columnare.挑战产生了部分抵抗力.
结论:
- 滑翔的运动性是Flavobacterium columnare.的一个重要的毒性因素.
- 这些发现突显了动力在柱状病的发病过程中的重要性.
- 这项研究表明,可以探索动力缺陷的F. columnare菌株,以开发鱼类疫苗或益生菌.
相关概念视频
Mechanism of Filopodia Formation
2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Cytoskeletal Proteins in Bacteria
3.4K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.4K
Intracellular Movement of Viruses and Bacteria
2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K
Mechanism of Lamellipodia Formation
2.6K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.6K
Actin Polymerization and Cell Motility
5.2K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.2K
Membrane Asymmetry Regulating Transporters
4.5K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.5K


