相关实验视频
Updated: Jun 30, 2025

05:59
Nasal Wipes for Influenza A Virus Detection and Isolation from Swine
Published on: December 4, 2015
9.4K
同感染和非随机重组推动了猪肠道冠状病毒的进化
Jiahui Guo1,2, Yinan Lai1,2, Zhixiang Yang1,2
1National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China.
Emerging microbes & infections
|March 22, 2024
概括
与多个猪肠道冠状病毒的共同感染推动了病毒的进化. 跨物种重组主要发生在同一个属内,在deltacoronavirus历史中发挥了作用.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子进化分子进化
- 病毒遗传学 病毒遗传学
背景情况:
- 与多种病毒的同时感染是常见的,并推动病毒的进化.
- 人们对物种间的病毒再组合的了解很少.
- 猪肠道冠状病毒为研究这些现象提供了一个模型.
研究的目的:
- 调查四种猪肠道冠状病毒之间的共感染和重组模式.
- 确定物种间重组在病毒进化中的作用.
- 为了探索deltacoronaviruses的重组史.为了探索deltacoronaviruses的重组史.
主要方法:
- 在4年内分析了4468个猪便和肠道组织样本.
- 确定共感染模式.
- 猪流行性腹病毒 (PEDV),传染性胃肠炎病毒 (TGEV),猪急性腹综合征冠状病毒 (SADS-CoV) 和猪三角冠状病毒 (PDCoV) 的重组分析.
主要成果:
- PEDV/PDCoV是最常见的共感染.
- 在PEDV/TGEV和SADS-CoV/TGEV之间检测到了重组事件,这表明了属内重组.
- 雀鸟被确定为deltacoronavirus重组中的独特宿主.
- 猪三角型冠状病毒 (PDCoV) 呈现出明显的重组史.
结论:
- 猪肠道冠状病毒是研究病毒共感染和再组合的有价值模型.
- 跨物种和内物种重组事件为冠状病毒演变提供了洞察力.
- 了解冠状病毒共感染和重组对于管理猪健康和公共健康至关重要.
相关概念视频
Viral Recombination
23.4K
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
23.4K
Viral Mutations
32.3K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
32.3K
Exon Recombination
3.6K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
3.6K
Types of Genetic Transfer Between Organisms
5.3K
5.3K
Conservative Site-specific Recombination and Phase Variation
6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.0K
Convergent Evolution
27.7K
Evolution shapes the features of organisms over time, ensuring that they are suited for the environments in which they live. Sometimes, selection pressure leads to the rise of similar but unrelated adaptations in organisms with no recent common ancestors, a process known as convergent evolution.
27.7K

