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肝炎E病毒感染人类丸组织和塞尔托利细胞
Tianxu Liu1, Yalei Cao2,3, Jiaming Weng2,3
1Department of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, People's Republic of China.
Emerging microbes & infections
|March 22, 2024
概括
肝炎E病毒 (HEV) 感染人类丸细胞,特别是塞尔托利细胞,并可能影响男性生殖健康. 塔克罗利斯治疗增强了HEV复制,为持续的病毒流失提供了洞察力.
科学领域:
- 病毒学 病毒学
- 生殖生物学 生殖生物学
- 免疫学 免疫学 免疫学
背景情况:
- 肝炎E病毒 (HEV) 感染在全球普遍存在.
- 射精中持续的HEV泄露表明生殖道复制,但缺少点器官和模型.
研究的目的:
- 为了确定人类丸中的HEV热带性.
- 调查HEV对男性生殖健康的潜在影响.
主要方法:
- 人类丸扩展体的ex vivo培养和初级人类塞尔托利细胞的体外培养.
- 用HEV基因型1和3 (HEV1,HEV3) 和HEV-C1.1进行注射.
- 感染人类细胞和HEV3感染的子丸的转录组分析.
主要成果:
- HEV3,但不是HEV1或HEV-C1,在人类丸扩展体和塞尔托利细胞中复制.
- 塔克罗利斯增强了HEV3复制,并使Sertoli细胞中的HEV1感染成为可能.
- 在子中,HEV3感染改变了细胞因子微环境,并降低了精子生成基因的调节.
结论:
- HEV感染人类丸组织和塞尔托利细胞,在塔克罗利斯治疗下增强复制.
- 这些发现提供了对射精中的HEV持久性的洞察,并提供了研究对策的ex vivo模型.
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