通过改变RRM2表达,GINS2促进了人类HNSCC的进展
Tianxiang Wang1,1, Luxi Qian1,1, Pingchuan Zhang1
1Department of Radiation Oncology, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Nanjing, Jiangsu, China.
Cancer biomarkers : section A of Disease markers
|March 22, 2024
概括
GINS2通过增加增殖和减少亡,可能通过RRM2改变,促进头部和部状细胞癌 (HNSCC) 的进展. 这表明GINS2是HNSCC的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- GINS2 (Go-No-Go复合体子单元2) 与各种癌症有关.
- 它在头部和部状细胞癌 (HNSCC) 中的特定作用和机制在很大程度上仍未被阐明.
研究的目的:
- 研究GINS2在HNSCC中的作用和潜在机制.
- 确定GINS2是否可以作为HNSCC.的潜在治疗点.
主要方法:
- 利用TCGA数据库用于HNSCC中的基因表达查.
- 采用免疫组织化学,qRT-PCR和西斑来评估GINS2的表达和功能.
- 进行细胞计数,MTT,殖民地形成和流动细胞计量试验,以评估增殖和亡.
- 进行生物信息学和微阵列分析,以确定GINS2.2的下游目标.
主要成果:
- 在HNSCC组织和细胞中,GINS2表达显著上调,与预后不佳相关.
- GINS2敲击抑制了HNSCC细胞增殖和诱导的亡.
- GINS2过度表达增强了繁殖和殖民地形成.
- 发现RRM2 (Ribonucleotide降解酶M2子单元) 表达调节了GINS2对增殖的影响.
结论:
- 这项研究是首次报告GINS2在HNSCC中的作用.
- 通过调节RRM2的表达,GINS2促进HNSCC的扩散,并抑制细胞亡.
- 在HNSCC治疗中,GINS2是一个有前途的治疗点.
相关概念视频
02:23Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
01:20The Retinoblastoma Gene
4.1K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
01:24Small GTPases - Ras and Rho
3.9K
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
3.9K
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K