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血管新素II对肠道微生物群的影响:与实验因素相比,影响很小
Rikeish R Muralitharan1,2,3, Michael E Nakai1, Matthew Snelson1,3
1Hypertension Research Laboratory, School of Biological Sciences, Faculty of Science, Monash University, 18 Innovation Walk, Clayton, 3800 Melbourne, Australia.
Cardiovascular research
|March 22, 2024
概括
ангиотензин II 对小鼠的肠道微生物群产生影响,但与饮食和采样地点等其他因素相比,这种影响很小. 考虑到这些变量对于可复制高血压研究至关重要.
科学领域:
- 微生物学 微生物学
- 动物模型 动物模型
- 高血压研究 高血压研究
背景情况:
- 用动物模型研究了肠道微生物组在高血压中的作用.
- 之前对血管素II对肠道微生物群的影响的研究规模较小.
- 实验因素可以影响微生物组的组成,影响研究的可重现性.
研究的目的:
- 为了确定血管素II对肠道微生物群的影响,在大型,多样化的小鼠队列中.
- 量化各种实验因素对微生物群变异的影响.
- 在高血压模型中评估微生物组特征的可复制性.
主要方法:
- 对来自303只C57BL/6J小鼠的538个胃肠道样本进行了回顾性分析.
- 16S rRNA基因测序 (V4区域) 的不同小鼠群 (基因型,饮食,设施,年龄).
- 统计分析,以评估血管素II和其他因素对微生物群多样性和组成的影响.
主要成果:
- ангиотензин II 治疗显著改变了α和β多样性 (分别P=0.0137和P<0.001).
- 观察到的模式表明,有益的短链脂肪酸产生细菌减少.
- 动物设施,饮食,基因型,年龄,性别和采样地点显著影响了微生物组;采样地点 (6.8%) 和饮食 (6%) 有最大的影响,而血管新素II (0.4%) 和性别 (0.4%) 有最小的影响.
结论:
- 大规模的数据证实血管素II会影响肠道微生物群,这与较小的研究相一致.
- 与其他因素相比, ангиотензин II 对微生物群的影响很小.
- 控制实验变量对于可复制和可翻译的临床前高血压研究至关重要.
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