在Drosophila melanogaster和D. simulans之间的辅助腺体转录组分歧的进化和遗传学
Alex C Majane1, Julie M Cridland1, Logan K Blair1
1Department of Evolution and Ecology, University of California, Davis, CA 95616, USA.
Genetics
|March 22, 2024
概括
在Drosophila杂交物中的调节分歧在辅助腺体中进行了研究. 跨差异比cis更常见,精液蛋白基因显示出高表达分歧,观察到有限的杂交误表.
科学领域:
- 进化遗传学的进化遗传学
- 基因调节 基因调节
- 德洛索菲拉的物种化发生.
背景情况:
- 杂交动物的基因特异表达 (ASE) 研究揭示了基因调控分歧和不相容性.
- 以前的Drosophila研究经常使用有限的组织采样 (例如,全身,生殖腺),可能缺少组织特异性的调节进化.
- 了解组织特定的监管分歧对于混合不兼容性的一般原则至关重要.
研究的目的:
- 描述Drosophila melanogaster和D. simulans之间F1杂交的辅助腺体中的调节进化和杂交错误表达.
- 调查与精液蛋白相关的基因中 cis 和 trans 调节差异的作用.
- 为了评估这种特定的体质男性生殖组织中错误表达的程度.
主要方法:
- 使用辅助腺体的转录组数据,分析F1杂交物中的等位基因特异性表达 (ASE).
- 类别之间的cis-和跨调节差异模式的比较.
- 利用ATAC-Seq检查染色质的可访问性及其与基因表达和ASE的相关性.
- 专注于编码精液蛋白质的基因.
主要成果:
- 变态作用的调节差异比 cis 作用的差异更为普遍,尽管大效应的变态差异很少见.
- 精液蛋白基因表现出显著更高的表达分歧,并受到cis和trans因子的调节.
- 与在Drosophila杂交物中研究的其他组织相比,辅助腺体显示出有限的基因误表.
- 在表达错误的基因中,男性偏向基因的比例过高,特别是表达不充分的基因.
- 通过ATAC-Seq测量的染色质可访问性,与物种间表达差异和混合ASE相关联.
结论:
- 草虫辅助腺体表现出独特的调节演变和混合误表的特征.
- 组织特异性ASE研究对于全面了解监管分歧和混合不兼容性至关重要.
- 精液蛋白基因是调控进化的热点,受到cis和trans机制的影响.
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