在生物打印研究中测量细胞增殖
Sophie Schweinitzer1,2, Masoumeh Jahani Kadousaraei2, Mehmet Serhat Aydin2
1Department of Biochemistry, Julius-Maximilians-University Würzburg, Würzburg, Germany.
Biomedical materials (Bristol, England)
|March 22, 2024
概括
在3D生物打印水凝中评估细胞增殖对于组织工程至关重要. 当前的方法在与生物墨水一起使用时显示出不准确性和差异,这表明需要进行多次测试以进行可靠的细胞增殖评估.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 细胞生物学 细胞生物学
背景情况:
- 细胞在水凝中的三维 (3D) 生物打印是组织工程和再生医学的关键.
- 对于这些结构来说,对封装细胞活力和增殖的可靠评估至关重要.
- 现有的增殖测定,为2D培养开发,可能不适合3D生物墨水.
研究的目的:
- 评估3D生物打印水凝的常见细胞增殖试验的适用性.
- 为了比较不同测定 (AlamarBlue,PicoGreen,视觉计数) 在各种生物墨水 (GelMA,酸) 中的准确性和一致性.
- 确定生物墨水和培养维度对增殖试验结果的影响.
主要方法:
- 人体纤维细胞被封装在凝甲基 (GelMA) 和酸水凝中,使用造和3D生物打印.
- 用AlamarBlue测定,PicoGreen DNA量化和视觉细胞计数来评估细胞增殖.
- 标准曲线是从二维培养和三维水凝生成的,用于数据推断.
主要成果:
- 当将2D衍生标准曲线的数据与3D水凝结果进行比较时,观察到潜在的不准确性.
- 在不同增殖试验中,细胞数量存在显著差异.
- 生物墨的选择 (GelMA与藻酸盐) 显著影响了扩散试验的结果.
结论:
- 针对2D培养物开发的标准增殖试验在3D生物打印结构上应用时可能会产生不准确的结果.
- 测试之间的差异凸显了基于生物墨水的细胞增殖研究中需要谨慎选择方法的需要.
- 建议应用多种评估方法,以更准确地评估组织工程应用的生物墨水中的细胞增殖.
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