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Updated: Jun 30, 2025

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
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Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Published on: March 18, 2012

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在混合QM/MM分子动力学模拟中的BLUF和LOV光受体蛋白中的flavin的氧化还原特性

Murat Kılıç1, Bernd Ensing1

  • 1Van't Hoff Institute for Molecular Sciences, University of Amsterdam, Park 904, Amsterdam 1098 XH, The Netherlands.

The journal of physical chemistry. B
|March 22, 2024
PubMed
概括

弗拉文辅因子是弗拉文的辅因子.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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