通过Cupriavidus necator使用氧化还原介质进行化学有机化的电发酵
André Gemünde1, Elena Rossini2, Oliver Lenz2
1Institute of Bioprocess Engineering and Pharmaceutical Technology and Competence Centre for Sustainable Engineering and Environmental Systems, University of Applied Sciences Mittelhessen, 35390 Giessen, Germany.
Bioelectrochemistry (Amsterdam, Netherlands)
|March 22, 2024
概括
库普里亚维杜斯 (Cupriavidus necator) 可以使用氧化还原介质进行阳极呼吸,在生物电化学系统中取代氧气. 铁化物实现了最高的电流密度,证明了其对高效微生物电合成的潜力.
科学领域:
- 微生物学 微生物学
- 生物电化学 生物电化学
- 生物技术是生物技术.
背景情况:
- 库普里亚维杜斯 (Cupriavidus necator) 是一种用于生物过程的多功能细菌,通常使用氧气作为终端电子受体.
- 微生物过程中的通风带来了泡,成本和安全问题等挑战,特别是和氧.
- 生物电化学系统通过使用电极作为终端电子接受器提供了替代方案,减轻了通风问题.
研究的目的:
- 建立从C. necator到电极的介导电子转移 (阳极呼吸).
- 用果糖作为电子和碳源来识别阳极呼吸的有效氧化还原介质.
- 评估C. necator生物电化学系统中各种氧化还原介质的功能和抑制作用.
主要方法:
- 在生物电化学系统中培养Cupriavidus necator.
- 测试11种常见的生物和非生物氧化还原介质用于电子转移.
- 测量电流密度和库伦比效率,以评估介质器的性能.
主要成果:
- 在C. necator和阳极之间成功建立了介导电子转移.
- 15mM的铁化物产生了最高的电流密度 (260.75μAcm-2).
- 用铁酸获得了64.1%的库伦比效率.
结论:
- 氧化还原介质对于使C. necator. 能够进行阳极呼吸至关重要.
- 在这种系统中,酸铁是阳极电子转移的高效调解剂.
- 这项研究为优化使用C. necator.生物电化学过程提供了基础.
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