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芯片上的生物发光生物传感器用于检测微生物表面污染

Reza Abbasi¹, Meruyert Imanbekova¹, Sebastian Wachsmann-Hogiu¹

¹Department of Bioengineering, McGill University, Montreal, QC, H3A 0E9, Canada.

Biosensors & bioelectronics

|March 22, 2024

概括

使用CMOS图像传感器的新型生物传感器通过ATP生物发光快速检测微生物污染. 这种具有成本效益的方法提供了与商业光度计可比的准确结果,用于监测表面卫生.

科学领域:

生物医学工程生物医学工程
微生物学微生物学
传感器技术传感器技术

背景情况:

传统的微生物检测方法缓慢而昂贵.
基于腺三酸盐 (ATP) 的生物发光为表面污染评估提供了更快的替代方案.
现有的ATP检测系统可能很昂贵,需要专门的设备.

研究的目的:

使用CMOS图像传感器开发和验证一种用于ATP检测的新型生物传感器.
评估CMOS生物传感器的性能,以量化表面上的微生物污染.
为了比较CMOS生物传感器的有效性与商业灯度计.

主要方法:

一个CMOS图像传感器通过移除镜头和IR过器进行了修改,用于直接样本分析.
超快速扫描器收集了表面样本,然后将其与化学发光试剂混合.
样品直接放置在CMOS传感器上,用于化学发光检测和分析.

主要成果:

CMOS生物传感器显示了10-1000 nM ATP的线性检测范围,检测极限为225 fmol.
与商业灯度计相比,观察到高相关性 (皮尔森 r = 0.98589) 和没有显著偏差.
CMOS生物传感器准确地反映了各种表面的微生物污染趋势,反映了商业仪器的结果.

结论:

一个基于CMOS图像传感器的生物传感器为ATP检测提供了一个高效和准确的平台.
这项技术为食品安全和卫生领域快速监测微生物污染提供了一个有希望的,潜在的低成本替代方案.
开发的生物传感器显示出在卫生评估环境中广泛应用的巨大潜力.

关键词:

这就是ATPATPATP ATP.生物发光是一种生物发光.这是一个CMOS图像传感器.食品安全食品安全微生物污染的发生

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