克里斯普尔-Cas12/Cas13:对其在传染病检测中的应用进行了文献识别分析和系统审查
Samson Leta1, Tesfaye Rufael Chibssa2, Jan Paeshuyse3
1Laboratory of Host Pathogen Interaction in Livestock, Division of Animal and Human Health Engineering, Department of Biosystems, KU Leuven, 3001 Leuven, Belgium; Department of Biomedical Sciences, College of Veterinary Medicine and Agriculture, Addis Ababa University, P.O. Box 34, Bishoftu, Ethiopia.
Journal of infection and public health
|March 22, 2024
概括
克里斯普尔-Cas12/Cas13研究激增,为170种病原体开发了580多种诊断试验. 这项技术对快速,在治疗点检测传染病有很大的前景.
科学领域:
- 在CRISPR-Cas12/Cas13研究中.
- 传染病诊断 传染病诊断 传染病诊断
背景情况:
- 传染病对全球健康和经济造成重大负担.
- CRISPR-Cas12/Cas13技术提供了新的诊断能力.
研究的目的:
- 检查CRISPR-Cas12/Cas13的当前研究环境.
- 分析CRISPR-Cas12/Cas13在传染病检测中的应用.
- 为了了解CRISPR-Cas12/Cas13研究的进化轨迹.
主要方法:
- 在2015-2022年期间对CRISPR-Cas12/Cas13篇文章进行了文献计量分析和系统审查.
- 从科学网络数据库中检索的数据.
- 在INPLASY注册的研究协议.
主要成果:
- 关于CRISPR-Cas12/Cas13的科学生产显示出高年增长率 (104.5%).
- 美国在出版物方面处于领先地位,580个针对170种病原体的诊断分析被确定,其中SARS-CoV-2是最受关注的.
- 重组酶聚合酶放大 (RPA) 和侧流测试占主导地位,其中72%的测试适合于临床检测.
结论:
- 研究的快速增长表明,在传染病诊断中,CRISPR-Cas12/Cas13具有很大的潜力.
- 克里斯普尔-卡斯技术在疾病检测方面具有优势.
- 克里斯普尔-Cas可能成为加速医疗点检测的关键技术.
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