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一个16个家族的碳水化合物结合模块 (CBM) 的特征和结构识别:对烯结合CBM的第一个结构洞察

Xuanwei Mei¹, Guanchen Liu¹, Guangning Chen¹

¹College of Food Science and Engineering, Ocean University of China, 1299 Sansha Road, Qingdao 266404, China.

International journal of biological macromolecules

|March 22, 2024

概括

研究人员发现了一种新的蛋白质AmCBM16,该蛋白质特别与,一个功能性多糖化合物结合. 这一发现为胺结合碳水化合物结合模块 (CBM) 提供了第一个结构洞察.

科学领域:

生物化学生物化学
结构生物学结构生物学
海洋微生物学海洋微生物学

背景情况:

波菲兰是来自Porphyra的一种功能性多糖,其线性结构由交替的α-l-galactopyranose-6-sulfate (L6S) 和β-d-galactopyranose (G) 单位组成.
碳水化合物结合模块 (CBM) 是多糖化物研究和应用的重要工具,但对素结合CBM的知识有限.
迄今为止,只发现了一种结合紫的CBM,缺乏结构信息.

研究的目的:

发现和描述具有烯特异性的新型CBM.
为了阐明一种新发现的烯结合CBM的结构.
了解参与CBM的甲酸识别中的分子相互作用.

主要方法:

从海洋细菌Aquimarina sp.中发现并表达了一种新型CBM16家族域. 在BL5.5中,我们可以看到BL5.
生物层干扰测试测试以确定复合蛋白AmCBM16对的结合亲和力和特异性.
进行X射线晶体学,以解析AmCBM16.16的三维结构.
位点定向突变发生,以确定参与蛋白质-碳水化合物相互作用的关键残留物.

主要成果:

一种新的CBM16蛋白,AmCBM16,成功地表达并证明了与烯的特定结合.
AmCBM16与兰四糖化物 (L6S-G) 2结合,其关联常数为1.3 × 103 M-1.1.
晶体结构显示AmCBM16具有由10个β-链组成的β-三明治折叠.
突变酶确定了Gly-30,Trp-31,Lys-88,Lys-123,Phe-125和Phe-127作为关键的结合残留物.

结论:

这项研究报告了AmCBM16的发现和表征,这是一种新的紫结合CBM.
由AmCBM16提供的结构洞察力代表了首次详细研究CBM识别氨酸的分子基础.
鉴定到的关键残留物为了解和设计用于相关应用的CBM提供了基础.

关键词:

碳水化合物结合模块的使用晶体结构晶体结构波菲兰是一种.

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