塞斯特林2和塞斯特林3保护精子发生,防止热引起的介质缺陷††
Wenhui Chen1,2,3, Mengchen Wang1,2,3, Huan Wang1,2,3
1Center for Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Biology of reproduction
|March 22, 2024
概括
雌激素2和3保护男性的生育能力免受热应激. 这些蛋白质的损失加剧了生殖细胞的损失,氧化应激和小鼠丸中介质失败,突出了它们在精子生成中的关键作用.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 氧化压力研究研究 氧化压力研究
背景情况:
- 热应激是男性不孕症的主要原因,原因是丸氧化应激和精子生成受损.
- 塞斯特林蛋白家族表现出抗氧化特性,但它们在丸热调节中的具体作用尚不清楚.
- 赛斯特林2和赛斯特林3的丸表达高于赛斯特林1.
研究的目的:
- 研究Sestrin2和Sestrin3在丸对热应激反应中的功能.
- 为了确定Sestrin2和Sestrin3缺乏对热应激条件下的精子生成的影响.
主要方法:
- 使用CRISPR/Cas9.9生成Sestrin2淘汰赛 (Sestrin2-/-) 和Sestrin3淘汰赛 (Sestrin3-/-) 的小鼠.
- 评估丸损伤,生殖细胞损失,氧化应激标志物和热暴露后野生类型和淘汰赛小鼠的亡.
- 精子细胞和染色体扩散的转录组分析,以评估介质完整性和DNA双链断裂修复.
主要成果:
- 与野生型对照相比,Sestrin2-/-和Sestrin3-/-小鼠在热应激后表现出明显更严重的丸损伤和生殖细胞损失.
- 缺乏Sestrin2或Sestrin3的小鼠表现出热引起的精子细胞亡的增加.
- 失落的Sestrin2和Sestrin3加剧了介质性失败,损害了热应激后的DNA双链断裂修复.
结论:
- 塞斯特林2和塞斯特林3在维持丸功能和精子生成中发挥着关键的保护作用,防止热应激诱导的损伤.
- 这些发现确定Sestrin2和Sestrin3是缓解因热等环境压力因素引起的男性不孕症的关键调解者.
相关概念视频
Meiosis II
45.6K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
45.6K
Spermatogenesis
102.4K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
102.4K
Meiosis I
40.6K
Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
40.6K
The Spindle Assembly Checkpoint
3.2K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.2K
Meiosis vs. Mitosis
55.5K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
55.5K
Separation of Sister Chromatids
3.6K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.6K


