一个真菌核心效应器利用OsPUX8B.2-OsCDC48-6模块来抑制植物免疫力
Xuetao Shi1,2, Xin Xie1, Yuanwen Guo1
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Nature communications
|March 23, 2024
概括
马格纳波特花 (Magnaporthe oryzae) 是一个很大的植物.
科学领域:
- 植物病原体相互作用
- 分子植物病理学
- 蛋白质质量控制机制 蛋白质质量控制机制
背景情况:
- 乌比基调节X (UBX) 域蛋白质是参与蛋白质质量控制的细胞分裂周期48 (CDC48) 的共因子.
- 在宿主-微生物相互作用中UBX蛋白质的作用尚不清楚.
- 麦格纳波特 (Magnaporthe oryzae) 是一种导致大米爆发病的真菌病原体.
研究的目的:
- 为了研究含有UBX的蛋白质在对Magnaporthe oryzae.的免疫力中的作用.
- 阐明真菌效应剂MoNLE1与大米蛋白相互作用的机制.
主要方法:
- 酵母两杂交测试以确定蛋白质相互作用.
- 同免疫沉以确认蛋白质结合.
- 定量实时PCR用于分析基因表达.
- 孔焦显微镜观察蛋白质局部化.
主要成果:
- 一个Magnaporthe oryzae效应剂MoNLE1通过准OsPUX8B.2.2来抑制大米免疫力.
- OsPUX8B.2的UBX域对其与OsATG8和OsCDC48-6的相互作用至关重要,通过26S蛋白质组调节其稳定性.
- OsPUX8B.2和OsCDC48-6增强了大米免疫力,而OsBHT则对抗爆发性真菌的防御进行了负面调节.
- MoNLE1诱导了OsPUX8B.2的降解,并破坏了它与OsBHT的相互作用.
结论:
- MoNLE1的毒性依赖于破坏OsPUX8B.2-OsCDC48-6复合体,导致免疫抑制.
- 设计抗MoNLE1的细胞内蛋白质可以在植物中赋予广泛而持久的抗爆能力.
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