光记者给出了对抗生素诱导的无稽之谈和框架转移错误翻译的新见解
Mariliis Hinnu1, Marta Putrinš2,3, Karin Kogermann3
1Institute of Technology, University of Tartu, 50411, Tartu, Estonia. mariliis.hinnu@ut.ee.
Scientific reports
|March 23, 2024
概括
我们开发了一种新的记者系统来测量细菌中的翻译错误. 大肠杆菌实验室菌株的误译率高于临床分离品,特别是在人体尿液中.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 细菌的转化忠实性对于细胞功能至关重要.
- 了解翻译错误是解读细菌适应和抗生素耐药性的关键.
研究的目的:
- 开发和验证一种新的报告系统,用于在单细胞水平上量化翻译错误.
- 为了比较实验室大肠杆菌菌株和临床分离物之间的错误翻译率.
- 研究环境条件和抗生素对转化忠实性的影响.
主要方法:
- 开发一个双光报告系统 (GFP和突变的mScarlet-I) 来检测蛋白质合成能力和翻译错误.
- 使用流细胞计和光显微镜进行单细胞分析.
- 在各种介质 (包括人体尿液) 中培养大肠杆菌,并将其暴露在抗生素 (阿米卡辛,阿齐思罗米辛) 中.
主要成果:
- 实验室大肠杆菌菌株显示出比临床分离物更高的误译率.
- 人体尿液的增长显著增加了尿病原性大肠杆菌的翻译框架转移.
- 艾米卡辛主要诱导停止-codon阅读,而阿齐思罗米辛在更高水平上诱导了移和停止-codon阅读.
- 由于潜在的蛋白质泄漏,抗生素治疗复杂了记者信号.
结论:
- 开发的报告员系统能够精确地测量细菌转化错误的单细胞测量.
- 环境因素,如人体尿液,可以加剧细菌的翻译错误.
- 不同类型的抗生素会引起不同类型和水平的转化错误,突出显示复杂的抗菌机制.
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